产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8488, RRID:AB_2840542.
展开/折叠
BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050;
抗原和靶标
- P49736 MCM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49736 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| S5 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| S7 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| T9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) | Uniprot |
| T25 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) | Uniprot |
| S31 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S32 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) , P24941 (CDK2) | Uniprot |
| S41 | Phosphorylation | O00311 (CDC7) , P24941 (CDK2) , P68400 (CSNK2A1) , P50613 (CDK7) | Uniprot |
| S53 | Phosphorylation | P68400 (CSNK2A1) , P24941 (CDK2) , P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| T59 | Phosphorylation | P50613 (CDK7) | Uniprot |
| Y81 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , Q13535 (ATR) , O00311 (CDC7) | Uniprot |
| R126 | Methylation | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) | Uniprot |
| T158 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S220 | Phosphorylation | O00311 (CDC7) , O14965 (AURKA) | Uniprot |
| K224 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| R307 | Methylation | Uniprot | |
| C315 | S-Nitrosylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K469 | Acetylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| R623 | Methylation | Uniprot | |
| R677 | Methylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| R751 | Methylation | Uniprot | |
| K752 | Ubiquitination | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot | |
| K863 | Ubiquitination | Uniprot | |
| K868 | Ubiquitination | Uniprot | |
| S878 | Phosphorylation | Uniprot | |
| K896 | Acetylation | Uniprot | |
| K896 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Nucleus.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.
Belongs to the MCM family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.