| 产品: | 磷酸化 NHE-3 (Ser555) 抗体 |
| 货号: | AF8495 |
| 描述: | Rabbit polyclonal antibody to Phospho-NHE-3 (Ser555) |
| 应用: | WB IHC |
| 反应: | Human, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 93 kD; 93kD(Calculated). |
| 蛋白号: | P48764 |
| RRID: | AB_2840549 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8495, RRID:AB_2840549.
展开/折叠
MGC126718; MGC126720; Na(+)/H(+) exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3; Solute carrier family 9 (sodium/hydrogen exchanger), member 3; Solute carrier family 9 member 3;
抗原和靶标
- P48764 SL9A3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWGLGARGPDRGLLLALALGGLARAGGVEVEPGGAHGESGGFQVVTFEWAHVQDPYVIALWILVASLAKIGFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVVGTVWNAATTGLSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLFIIVFGESLLNDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFTKHVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASSAETIIFMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMVQLEPIDQVVLSYGGLRGAVAFALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQGLTIKPLVQWLKVKRSEHREPRLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSHFDRKFLSRVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNVVNVDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHELTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKRERAQKRRNSSIPNGKLPMESPAQNFTIKEKDLELSDTEEPPNYDEEMSGGIEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLARLPPWLSPGETVVPSQRARTQIPYSPGTFCRLMPFRLSSKSVDSFLQADGPEERPPAALPESTHM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48764 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y396 | Phosphorylation | Uniprot | |
| Y502 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| T641 | Phosphorylation | Uniprot | |
| S663 | Phosphorylation | P51812 (RPS6KA3) , O00141 (SGK1) | Uniprot |
| K682 | Acetylation | Uniprot | |
| S794 | Phosphorylation | Uniprot |
研究背景
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane.
Apical cell membrane>Multi-pass membrane protein.
Note: In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression (By similarity).
Binds SLC9A3R1 and SLC9A3R2. Interacts with CHP1, CHP2 and SHANK2. Interacts with PDZK1 (via C-terminal PDZ domain) (By similarity). Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Interacts with AHCYL1; the interaction is required for SLC9A3 activity.
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
研究领域
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
· Organismal Systems > Digestive system > Protein digestion and absorption.
· Organismal Systems > Digestive system > Mineral absorption.
限制条款
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