Phospho-PDE3A (Ser312) Antibody - #AF8501

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产品: 磷酸化 PDE3A (Ser312) 抗体
货号: AF8501
描述: Rabbit polyclonal antibody to Phospho-PDE3A (Ser312)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Rabbit, Chicken
分子量: 125 kDa; 125kD(Calculated).
蛋白号: Q14432
RRID: AB_2840555

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-PDE3A (Ser312) Antibody detects endogenous levels of PDE3A only when phosphorylated at Ser312.
RRID:
AB_2840555
引用格式: Affinity Biosciences Cat# AF8501, RRID:AB_2840555.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5''-cyclic phosphodiesterase A; cAMP phosphodiesterase myocardial cGMP inhibited; CGI PDE; CGI PDE A; CGI-PDE A; cGMP inhibited 3' 5' cyclic phosphodiesterase A; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; Human cGMP inhibited cAMP phosphodiesterase mRNA; PDE 3A; Pde3a; PDE3A_HUMAN; Phosphodiesterase 3A; Phosphodiesterase 3A cGMP inhibited;

抗原和靶标

免疫原:
Uniprot:

Q14432(PDE3A_HUMAN) >>Visit HPA database.

基因/基因ID:
PDE3A(5139)
序列:
MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSLSFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLLCAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRLGVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Chicken
100
Rabbit
100
Zebrafish
83
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14432 作为底物

Site PTM Type Enzyme
K287 Ubiquitination
S292 Phosphorylation P17252 (PRKCA)
S293 Phosphorylation P17252 (PRKCA)
S294 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
S312 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
T399 Phosphorylation
S400 Phosphorylation
S402 Phosphorylation
S408 Phosphorylation
S410 Phosphorylation
S413 Phosphorylation
K418 Ubiquitination
S428 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
S438 Phosphorylation P17252 (PRKCA)
S439 Phosphorylation
T440 Phosphorylation
T442 Phosphorylation
T443 Phosphorylation
T444 Phosphorylation
T445 Phosphorylation
S463 Phosphorylation
S465 Phosphorylation P17252 (PRKCA)
K467 Ubiquitination
S475 Phosphorylation
S478 Phosphorylation
K489 Ubiquitination
S492 Phosphorylation P17252 (PRKCA)
T494 Phosphorylation
Y497 Phosphorylation
K506 Ubiquitination
K518 Ubiquitination
S520 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
S527 Phosphorylation
S528 Phosphorylation
T533 Phosphorylation
K541 Ubiquitination
S550 Phosphorylation
T553 Phosphorylation
T554 Phosphorylation
K556 Ubiquitination
S558 Phosphorylation
S561 Phosphorylation
T568 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation
T580 Phosphorylation
S593 Phosphorylation
S613 Phosphorylation
T615 Phosphorylation
T618 Phosphorylation
T643 Phosphorylation
S654 Phosphorylation
K729 Ubiquitination
K912 Ubiquitination
K919 Ubiquitination
K923 Ubiquitination
K947 Ubiquitination
K956 Ubiquitination
K1088 Ubiquitination
S1103 Phosphorylation
T1107 Phosphorylation
K1120 Ubiquitination
T1136 Phosphorylation

研究背景

功能:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Endocrine system > Renin secretion.

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