| 产品: | 磷酸化 PNKP (Ser114) 抗体 |
| 货号: | AF8503 |
| 描述: | Rabbit polyclonal antibody to Phospho-PNKP (Ser114) |
| 应用: | WB |
| 反应: | Human |
| 分子量: | 60 kDa; 57kD(Calculated). |
| 蛋白号: | Q96T60 |
| RRID: | AB_2840557 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8503, RRID:AB_2840557.
展开/折叠
2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; DNA 5''-kinase/3''-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3' phosphatase (PNKP); MCSZ; PNK 1; PNK1; Pnkp; PNKP DNA kinase; PNKP_HUMAN; Polynucleotide 3'-phosphatase; Polynucleotide 5' hydroxyl kinase; Polynucleotide 5''-hydroxyl-kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; Polynucleotide kinase-3''-phosphatase;
抗原和靶标
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
- Q96T60 PNKP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG
翻译修饰 - Q96T60 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Q13315 (ATM) , P78527 (PRKDC) | Uniprot |
| T118 | Phosphorylation | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | P78527 (PRKDC) , Q13315 (ATM) | Uniprot |
| K142 | Ubiquitination | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K183 | Sumoylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| R224 | Methylation | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot |
研究背景
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
Nucleus.
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver.
Monomer.
The FHA domain binds threonine-phosphorylated peptides from XRCC1/4, and is responsible for the recruitment of PNKP to the sites of DNA repair. The affinity is ten times greater if peptides are also phosphorylated on the serine preceeding the phosphothreonine.
In the N-terminal section; belongs to the DNA 3' phosphatase family.
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