产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8517, RRID:AB_2840571.
展开/折叠
DNA topoisomerase 2 binding protein 1; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II binding protein 1; DNA topoisomerase II binding protein; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; DNA topoisomerase IIbeta binding protein 1; Hypothetical protein KIAA0259 [Fragment]; KIAA0259; TOP2BP1; TOPB1_HUMAN; TOPBP 1; TopBP1; Topoisomerase (DNA) II binding protein 1; Topoisomerase II binding protein 1;
抗原和靶标
- Q92547 TOPB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRNDKEPFFVKFLKSSDNSKCFFKALESIKEFQSEEYLQIITEEEALKIKENDRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSESICNSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECFSKGYMLSEEPYIHANYQPVEIPVSHKPESKAALLKKKNSSFSKKDFAPSEKHEQADEDLLSQYENGSSTVVEAKTSEARPFNDSTHAEPLNDSTHISLQEENQSSVSHCVPDVSTITEEGLFSQKSFLVLGFSNENESNIANIIKENAGKIMSLLSRTVADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKSNPLFTPVPVMTGMTPLEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLIENSTKEERSLETEITNGINLNSDTAEHPGTRLQTHRKTVVTPLDMNRFQSKAFRAVVSQHARQVAASPAVGQPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGRPSQQKRKPSTPLSEVIVKNLQLALANSSRNAVALSASPQLKEAQSEKEEAPKPLHKVVVCVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRPNDTNREYKSVKERGVHIVSEHWLLDCAQECKHLPESLYPHTYNPKMSLDISAVQDGRLCNSRLLSAVSSTKDDEPDPLILEENDVDNMATNNKESAPSNGSGKNDSKGVLTQTLEMRENFQKQLQEIMSATSIVKPQGQRTSLSRSGCNSASSTPDSTRSARSGRSRVLEALRQSRQTVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSCPTQYSELQVDIQNLEDSPFQKPLHDSEIAKQAVCDPGNIRVTEAPKHPISEELETPIKDSHLIPTPQAPSIAFPLANPPVAPHPREKIITIEETHEELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLPGHSVPLFKEATHLFSDLNKLKPDDSGVNIAEAAAQNVYCLRTEYIADYLMQESPPHVENYCLPEAISFIQNNKELGTGLSQKRKAPTEKNKIKRPRVH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92547 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S492 | Phosphorylation | Q13315 (ATM) | Uniprot |
| Y494 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K581 | Acetylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| T618 | Phosphorylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| K743 | Ubiquitination | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| T776 | Phosphorylation | Uniprot | |
| T779 | Phosphorylation | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| T822 | Phosphorylation | Uniprot | |
| S836 | Phosphorylation | Uniprot | |
| K840 | Ubiquitination | Uniprot | |
| T848 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S853 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K856 | Ubiquitination | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| T861 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| S878 | Phosphorylation | Uniprot | |
| S879 | Phosphorylation | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| S888 | Phosphorylation | Uniprot | |
| S998 | Phosphorylation | Uniprot | |
| S1002 | Phosphorylation | Uniprot | |
| S1012 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| K1058 | Sumoylation | Uniprot | |
| K1058 | Ubiquitination | Uniprot | |
| T1062 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T1064 | Phosphorylation | Uniprot | |
| S1093 | Phosphorylation | Uniprot | |
| C1099 | S-Nitrosylation | Uniprot | |
| S1126 | Phosphorylation | Uniprot | |
| S1138 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1159 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S1183 | Phosphorylation | Uniprot | |
| S1192 | Phosphorylation | Uniprot | |
| S1216 | Phosphorylation | Uniprot | |
| T1221 | Phosphorylation | Uniprot | |
| T1231 | Phosphorylation | Uniprot | |
| K1253 | Ubiquitination | Uniprot | |
| K1265 | Ubiquitination | Uniprot | |
| K1289 | Ubiquitination | Uniprot | |
| K1297 | Ubiquitination | Uniprot | |
| K1317 | Ubiquitination | Uniprot | |
| K1412 | Methylation | Uniprot | |
| K1421 | Ubiquitination | Uniprot | |
| K1432 | Ubiquitination | Uniprot | |
| K1443 | Ubiquitination | Uniprot | |
| K1445 | Ubiquitination | Uniprot | |
| S1504 | Phosphorylation | Uniprot | |
| K1506 | Ubiquitination | Uniprot |
研究背景
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Chromosome.
Note: Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
Highly expressed in heart, brain, placenta, lung and kidney.
Interacts with POLE. Interacts with RAD9A. Interacts with UBR5. Interacts with E2F1. Interacts with PML. Interacts with SMARCA2. Interacts with SMARCA4. Interacts with RHNO1. May interact with TOP2B. Interacts with TICRR. Interacts with HELB.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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