Cofilin Antibody - #AF0761

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产品: Cofilin 抗体
货号: AF0761
描述: Rabbit polyclonal antibody to Cofilin
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 19kDa; 19kD(Calculated).
蛋白号: Q9Y281 | P23528
RRID: AB_2834371

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Cofilin Antibody detects endogenous levels of total Cofilin.
RRID:
AB_2834371
引用格式: Affinity Biosciences Cat# AF0761, RRID:AB_2834371.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CFL 2; CFL2; COF2_HUMAN; Cofilin 2 muscle; Cofilin; Cofilin muscle; Cofilin muscle isoform; Cofilin-2; Cofilin2; muscle isoform; NEM 7; NEM7; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18;

抗原和靶标

免疫原:
Uniprot:

Q9Y281(COF2_HUMAN) >>Visit HPA database.

P23528(COF1_HUMAN) >>Visit HPA database.

基因/基因ID:
CFL2(1073) | CFL1(1072)
表达:
Q9Y281 COF2_HUMAN:

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

P23528 COF1_HUMAN:

Widely distributed in various tissues.

描述:
cofilin 1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described.
序列:
MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y281/P23528 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation Q15569 (TESK1) , Q96S53 (TESK2) , P53667 (LIMK1)
K13 Ubiquitination
K19 Acetylation
K19 Methylation
K19 Sumoylation
K19 Ubiquitination
S23 Phosphorylation
S24 Phosphorylation
K44 Acetylation
Y68 Phosphorylation
K73 Ubiquitination
Y82 Phosphorylation
Y85 Phosphorylation
T88 Phosphorylation
Y89 Phosphorylation
T91 Phosphorylation
K92 Acetylation
K92 Ubiquitination
S94 Phosphorylation
K95 Ubiquitination
K96 Acetylation
K96 Methylation
K96 Ubiquitination
S108 Phosphorylation
K112 Sumoylation
K112 Ubiquitination
K114 Acetylation
K114 Sumoylation
K114 Ubiquitination
Y117 Phosphorylation
S119 Phosphorylation
S120 Phosphorylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
K125 Acetylation
T129 Phosphorylation
K144 Ubiquitination
S160 Phosphorylation
Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation P53671 (LIMK2) , P53667 (LIMK1) , Q13418 (ILK) , Q15139 (PRKD1) , Q96S53 (TESK2) , Q15569 (TESK1) , Q7Z2Y5 (NRK)
S8 Phosphorylation
K13 Acetylation
K13 Sumoylation
K13 Ubiquitination
K19 Acetylation
K19 Methylation
K19 Sumoylation
K19 Ubiquitination
K22 Ubiquitination
S23 Phosphorylation P17252 (PRKCA)
S24 Phosphorylation P17252 (PRKCA)
T25 Phosphorylation
K30 Acetylation
K30 Sumoylation
K30 Ubiquitination
K31 Acetylation
K33 Acetylation
K34 Sumoylation
K34 Ubiquitination
C39 S-Nitrosylation
S41 Phosphorylation
K44 Acetylation
K44 Sumoylation
K44 Ubiquitination
K45 Acetylation
K45 Sumoylation
K45 Ubiquitination
K53 Sumoylation
K53 Ubiquitination
T63 Phosphorylation
Y68 Phosphorylation P12931 (SRC)
T70 Phosphorylation
K73 Acetylation
K73 Ubiquitination
K78 Acetylation
K78 Ubiquitination
C80 S-Nitrosylation
Y82 Phosphorylation
Y85 Phosphorylation
T88 Phosphorylation
Y89 Phosphorylation
T91 Phosphorylation
K92 Acetylation
K92 Ubiquitination
S94 Phosphorylation
K95 Ubiquitination
K96 Acetylation
K96 Methylation
K96 Ubiquitination
S108 Phosphorylation
K112 Sumoylation
K112 Ubiquitination
K114 Acetylation
K114 Sumoylation
K114 Ubiquitination
Y117 Phosphorylation
S119 Phosphorylation
S120 Phosphorylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
K125 Acetylation
K126 Acetylation
K127 Acetylation
K127 Ubiquitination
T129 Phosphorylation
K132 Acetylation
K132 Sumoylation
K132 Ubiquitination
C139 S-Nitrosylation
Y140 Phosphorylation
K144 Acetylation
K144 Ubiquitination
C147 S-Nitrosylation
K152 Ubiquitination
S156 Phosphorylation
S160 Phosphorylation
K164 Sumoylation
K164 Ubiquitination

研究背景

功能:

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).

翻译修饰:

The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

细胞定位:

Nucleus matrix. Cytoplasm>Cytoskeleton.
Note: Colocalizes with CSPR3 in the Z line of sarcomeres.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

亚基结构:

Interacts with CSRP3; possibly two molecules of CFL2 can interact with one molecule if CSRP3.

蛋白家族:

Belongs to the actin-binding proteins ADF family.

功能:

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity).

翻译修饰:

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

细胞定位:

Nucleus matrix. Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium.
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely distributed in various tissues.

亚基结构:

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.

蛋白家族:

Belongs to the actin-binding proteins ADF family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). Circ_0008673 regulates breast cancer malignancy by miR-153-3p/CFL2 axis. ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 2022 (PubMed: 34324029) [IF=2.6]

Application: WB    Species: Human    Sample: breast tissues

Fig. 3 MiR-153-3p regulated breast cancer cell processes by binding to CFL2. A Dual-luciferase reporter assay was performed to demonstrate that miR-153-3p was directly associated with CFL2 in MCF-10AT and MCF-7 cells. B and C The protein level of CFL2 was detected by western blot in 42 pairs of breast cancer and paracancerous normal breast tissues as well as MCF-10A, MCF-10AT and MCF-7 cells. D–I MCF-10AT cells were transfected with in-miRNC, in-miR-153-3p, in-miR-153-3p+si-NC and in-miR-153-3p+si CFL2, respectively. D Cell viability was determined by CCK-8 assay. E Cell colony-forming ability was detected by cell colony-formation assay. F Cell apoptosis was analyzed by fow cytometry analysis. G and H Cell migratory and invasive capacities were determined by transwell assay. I The protein levels of E-cadherin and N-cadherin were determined by western blot analysis. A: adjacent normal breast tissues. T: breast cancer tissues. **P<0.01 and ***P<0.001

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