产品: 磷酸化 ACK1 (Tyr857/Tyr858)[Tyr859/Tyr860] 抗体
货号: DF2998
描述: Rabbit polyclonal antibody to Phospho-ACK1 (Tyr857/Tyr858)[Tyr859/Tyr860]
应用: WB IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 115KD; 115kD(Calculated).
蛋白号: Q07912
RRID: AB_2840977

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-ACK1 (Tyr857/Tyr858) Antibody detects endogenous levels of ACK1 only when phosphorylated at Tyr859/Tyr860, which site historically referenced as Tyr857/Tyr859.
RRID:
AB_2840977
引用格式: Affinity Biosciences Cat# DF2998, RRID:AB_2840977.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acetate kinase 1; Acetokinase 1; ACK 1; ACK; ACK-1; ACK1; ACK1_HUMAN; Activated Cdc42 associated kinase 1; Activated CDC42 kinase 1; Activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK 2; TNK2; Tyrosine kinase non receptor 2; Tyrosine kinase non receptor protein 2; Tyrosine kinase non-receptor protein 2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q07912 ACK1_HUMAN:

The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.

序列:
MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPPQHLGGVKKPTYDPVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPSARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCAPSLAQLAMDACSLLDETPPQSPTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVPEQARPPPPLEDNLFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQAPAGSPAPSPSPGGDDKPQVPPRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAGPCILPIVRDGKKVSSTHYYLLPERPSYLERYQRFLREAQSPEEPTPLPVPLLLPPPSTPAPAAPTATVRPMPQAALDPKANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMAMVHGVTTEECQAALQCHGWSVQRAAQYLKVEQLFGLGLRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q07912 作为底物

Site PTM Type Enzyme
T33 Phosphorylation
S36 Phosphorylation
Y40 Phosphorylation
K48 Ubiquitination
S74 Phosphorylation
S77 Phosphorylation
S81 Phosphorylation
K100 Ubiquitination
T101 Phosphorylation
S102 Phosphorylation
K124 Ubiquitination
K131 Ubiquitination
S136 Phosphorylation
S149 Phosphorylation
Y193 Phosphorylation
T205 Phosphorylation
S212 Phosphorylation
Y284 Phosphorylation Q07912 (TNK2) , P12931 (SRC)
T465 Phosphorylation
K515 Ubiquitination
T517 Phosphorylation
Y518 Phosphorylation Q07912 (TNK2)
K533 Ubiquitination
T603 Phosphorylation
S607 Phosphorylation
Y635 Phosphorylation P09619 (PDGFRB)
S724 Phosphorylation
S728 Phosphorylation
S730 Phosphorylation
S774 Phosphorylation
S785 Phosphorylation
S789 Phosphorylation
S810 Phosphorylation
T818 Phosphorylation
K826 Acetylation
Y827 Phosphorylation Q07912 (TNK2)
T829 Phosphorylation
R839 Methylation
S855 Phosphorylation
S856 Phosphorylation
T857 Phosphorylation
Y859 Phosphorylation Q07912 (TNK2) , P51813 (BMX)
Y860 Phosphorylation P51813 (BMX)
S867 Phosphorylation
Y868 Phosphorylation
Y872 Phosphorylation Q07912 (TNK2)
S881 Phosphorylation
T925 Phosphorylation
R933 Methylation
K1011 Ubiquitination
K1037 Ubiquitination

翻译修饰 - Q07912 作为激酶

Substrate Site Source
P10275 (AR) Y269 Uniprot
P10275 (AR) Y365 Uniprot
P31749 (AKT1) Y176 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P42768 (WAS) S277 Uniprot
P42768 (WAS) Y291 Uniprot
P56945 (BCAR1) Y165 Uniprot
P56945 (BCAR1) Y249 Uniprot
P56945 (BCAR1) Y410 Uniprot
Q07912 (TNK2) Y284 Uniprot
Q07912 (TNK2) Y518 Uniprot
Q07912 (TNK2) Y827 Uniprot
Q07912 (TNK2) Y859 Uniprot
Q07912 (TNK2) Y872 Uniprot
Q9NZC7 (WWOX) Y287 Uniprot
Q9Y4C1 (KDM3A) Y1114 Uniprot
Q9Y5X1 (SNX9) Y56 Uniprot

研究背景

功能:

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

翻译修饰:

Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits.

Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity).

细胞定位:

Cell membrane. Nucleus. Endosome. Cell junction>Adherens junction. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle. Membrane>Clathrin-coated pit. Cytoplasm>Perinuclear region.
Note: The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Colocalizes with EGFR on endosomes. Nuclear translocation is CDC42-dependent.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.

亚基结构:

Interacts with NEDD4 (via WW3 domain). NEDD4L and EGF promote association with NEDD4 (By similarity). Homodimer. Interacts with AR, CDC42, WWASL and WWOX. Interacts with CSPG4 (activated). Interacts with MERTK (activated); stimulates autophosphorylation. May interact (phosphorylated) with HSP90AB1; maintains kinase activity. Interacts with NPHP1. Interacts with SNX9 (via SH3 domain). Interacts with SRC (via SH2 and SH3 domain). Interacts with EGFR, and this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts (via kinase domain) with AKT1. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with BCAR1/p130cas via SH3 domains. Forms complexes with GRB2 and numerous receptor tyrosine kinases (RTK) including LTK, AXL or PDGFRL, in which GRB2 promotes RTK recruitment by TNK2.

蛋白家族:

The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.

The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.

The UBA domain binds both poly- and mono-ubiquitin.

Belongs to the protein kinase superfamily. Tyr protein kinase family.

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