Phospho-IRAK4 (Thr345/Ser346) Antibody - #DF7567
(2)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7567, RRID:AB_2841060.
展开/折叠
IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;
抗原和靶标
- Q9NWZ3 IRAK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NWZ3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| T6 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S8 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K33 | Ubiquitination | Uniprot | |
| K34 | Acetylation | Uniprot | |
| S114 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K115 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S151 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S152 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S157 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T209 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K313 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T342 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T345 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| S346 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| T352 | Phosphorylation | Q9NWZ3 (IRAK4) | Uniprot |
| K400 | Sumoylation | Uniprot | |
| K416 | Ubiquitination | Uniprot |
翻译修饰 - Q9NWZ3 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P14598 (NCF1) | T133 | Uniprot |
| P14598 (NCF1) | T138 | Uniprot |
| P14598 (NCF1) | T153 | Uniprot |
| P14598 (NCF1) | S288 | Uniprot |
| P14598 (NCF1) | S320 | Uniprot |
| P14598 (NCF1) | S345 | Uniprot |
| P14598 (NCF1) | S348 | Uniprot |
| P14598 (NCF1) | T356 | Uniprot |
| P14598 (NCF1) | S359 | Uniprot |
| P14598 (NCF1) | S370 | Uniprot |
| P51617-4 (IRAK1) | S376 | Uniprot |
| P51617-1 (IRAK1) | T387 | Uniprot |
| P58753 (TIRAP) | T28 | Uniprot |
| Q96FA3 (PELI1) | S70 | Uniprot |
| Q96FA3 (PELI1) | S76 | Uniprot |
| Q96FA3 (PELI1) | S78 | Uniprot |
| Q96FA3 (PELI1) | T80 | Uniprot |
| Q96FA3 (PELI1) | S82 | Uniprot |
| Q96FA3 (PELI1) | T86 | Uniprot |
| Q96FA3 (PELI1) | T127 | Uniprot |
| Q96FA3 (PELI1) | T288 | Uniprot |
| Q96FA3 (PELI1) | S293 | Uniprot |
| Q9NWZ3 (IRAK4) | T6 | Uniprot |
| Q9NWZ3 (IRAK4) | S8 | Uniprot |
| Q9NWZ3 (IRAK4) | S114 | Uniprot |
| Q9NWZ3 (IRAK4) | S150 | Uniprot |
| Q9NWZ3 (IRAK4) | S151 | Uniprot |
| Q9NWZ3 (IRAK4) | S152 | Uniprot |
| Q9NWZ3 (IRAK4) | T208 | Uniprot |
| Q9NWZ3 (IRAK4) | T209 | Uniprot |
| Q9NWZ3 (IRAK4) | T342 | Uniprot |
| Q9NWZ3 (IRAK4) | T345 | Uniprot |
| Q9NWZ3 (IRAK4) | S346 | Uniprot |
| Q9NWZ3 (IRAK4) | T352 | Uniprot |
研究背景
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Phosphorylated.
Cytoplasm.
Associates with MYD88 and IRAK2 to form a ternary complex called the Myddosome. Once phosphorylated, IRAK4 dissociates from the receptor complex and then associates with the TNF receptor-associated factor 6 (TRAF6), IRAK1, and PELI1; this intermediate complex is required for subsequent NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Interacts with IL1RL1.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
文献引用
Application: WB Species: Human Sample: HCT‐116 cells
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