Phospho-IKB epsilon (Ser161) Antibody - #AF3002
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3002, RRID:AB_2834441.
展开/折叠
I kappa B epsilon; I-kappa-B-epsilon; IkappaBepsilon; IkB E; IkB-E; IkB-epsilon; IKBE; IKBE_HUMAN; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35, member B2;
抗原和靶标
Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
- O00221 IKBE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNQRRSESRPGNHRLQAYAEPGKGDSGGAGPLSGSARRGRGGGGAIRVRRPCWSGGAGRGGGPAWAVRLPTVTAGWTWPALRTLSSLRAGPSEPHSPGRRPPRAGRPLCQADPQPGKAARRSLEPDPAQTGPRPARAAGMSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTYTLSLLGGPEAEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALHLAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQHLACARCLLEGRPEPGRGTSHSLDLQLQNWQGLACLHIATLQKNQPLMELLLRNGADIDVQEGTSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00221 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S54 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot |
研究背景
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Serine phosphorylated; followed by proteasome-dependent degradation.
Cytoplasm.
Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.
Belongs to the NF-kappa-B inhibitor family.
研究领域
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
限制条款
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