Phospho-MITF (Ser180) Antibody - #AF3027

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产品: 磷酸化 MITF (Ser180) 抗体
货号: AF3027
描述: Rabbit polyclonal antibody to Phospho-MITF (Ser180)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
分子量: 50~80kDa; 59kD(Calculated).
蛋白号: O75030
RRID: AB_2834458

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Phospho-MITF (Ser180) Antibody detects endogenous levels of MITF only when phosphorylated at Serine 180.
RRID:
AB_2834458
引用格式: Affinity Biosciences Cat# AF3027, RRID:AB_2834458.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BHLHE32; bHLHe32; Class E basic helix-loop-helix protein 32; CMM8; Homolog of mouse microphthalmia; Mi; Microphthalmia associated transcription factor; Microphthalmia, mouse, homolog of; Microphthalmia-associated transcription factor; MITF; MITF_HUMAN; mitfa; nacre; WS2; WS2A; z3A.1;

抗原和靶标

免疫原:
Uniprot:

O75030(MITF_HUMAN) >>Visit HPA database.

基因/基因ID:
MITF(4286)
表达:
O75030 MITF_HUMAN:

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

描述:
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
序列:
MQSESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSSSAEHPGASKPPISSSSMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVPVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75030 作为底物

Site PTM Type Enzyme
T54 Phosphorylation
S55 Phosphorylation
K79 Sumoylation
K128 Ubiquitination
K140 Sumoylation
S157 Phosphorylation
S180 Phosphorylation P28482 (MAPK1)
K198 Ubiquitination
S216 Phosphorylation
K289 Sumoylation
S301 Phosphorylation
K308 Ubiquitination
R330 Methylation
K350 Ubiquitination
S357 Phosphorylation
Y360 Phosphorylation
K372 Ubiquitination
S405 Phosphorylation P49840 (GSK3A)
S409 Phosphorylation Q15418 (RPS6KA1)
S414 Phosphorylation Q16539 (MAPK14)
K423 Sumoylation
T489 Phosphorylation
S491 Phosphorylation
S502 Phosphorylation
S504 Phosphorylation
S508 Phosphorylation
T510 Phosphorylation
S511 Phosphorylation
S512 Phosphorylation
S515 Phosphorylation
S516 Phosphorylation

研究背景

功能:

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

翻译修饰:

Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome.

Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

亚基结构:

Homodimer or heterodimer; dimerization is mediated via the coiled coil region. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA in the form of homodimer or heterodimer with either TFE3, TFEB or TFEC. Interacts with KARS1. Identified in a complex with HINT1 and CTNNB1. Interacts with VSX2 (By similarity).

蛋白家族:

The leucine zipper region is part of a larger coiled coil.

Belongs to the MiT/TFE family.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

文献引用

1). TGFβ2 Upregulates Tyrosinase Activity through Opsin-3 in Human Skin Melanocytes In Vitro. Journal of Investigative Dermatology, 2021 (PubMed: 34029574) [IF=6.5]
2). The genetic basis and potential molecular mechanism of yellow-albino northern snakehead (Channa argus). Open Biology, 2023 (PubMed: 36789536) [IF=5.8]

Application: WB    Species: snake    Sample:

Figure 5. The comparison of core melanogenesis genes in skins between WT and YM snakeheads. (a) Western blotting detection of TYR protein levels in skins of WT and YM snakeheads (mean ± s.e.m., n = 4). (b) qPCR detection of mRNA levels of the melanogenic genes (tyr, tyrp1a, tyrp1b, dct and pmel) at different ontogenetic stages (mean ± s.e.m., n = 3). (c) qPCR detection of mRNA levels of mitfa and mitfb in skins of WT and YM snakeheads (mean ± s.e.m., n = 3). (d) Western blotting detection of phospho-MITF(Ser180) protein levels in skins of WT and YM snakeheads (mean ± s.e.m., n = 4). The western blots are cropped and blot source data are available in the electronic supplementary material, figures S3 and S4. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s.: not significant.
3). Cistanche deserticola polysaccharide induces melanogenesis in melanocytes and reduces oxidative stress via activating NRF2/HO-1 pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32096914) [IF=5.3]

Application: WB    Species: human    Sample: HEMs

FIGURE 2|CDP promotes melanogenesis in HEMs. The HEMs were treated with CDP at different concentrations (20, 40 and 80 μg/mL) or medium alone (NC) for 48 h, then we observed the melanin by Fontana-Masson staining, measured the melanin content (OD value, 470 nm) by NaOH assay, and measured the tyrosinase activity (OD value, 475 nm) by tyrosinase activity assay; meanwhile, we measured the protein levels of MITF, p-MITF, TYR, TRP1, TRP2, RAB27A and FSCN1 by Western blotting and measure the grey value of protein bands by Image J: (A) melanin staining; (B) the melanin content; (C) the tyrosinase activity; (D) the protein levels of melanogenesis-related genes
4). p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis. Fish & Shellfish Immunology, 2019 (PubMed: 31669282) [IF=4.7]

Application: WB    Species: clams    Sample: haemocytes

Fig. 8.| (A) Phosphorylated MpMITF (p-MITF) expression in the haemocytes of M. petechialis in the control group and p38 inhibitor group, as measured by Western blot. β-actin was used as the internal reference to normalize the total protein quantity between samples.
5). Exploring the mechanism of Liuwei Dihuang formula for promoting melanin synthesis in juvenile zebrafish based on network pharmacology and molecular docking. Heliyon, 2023 (PubMed: 38027573) [IF=4.0]

Application: WB    Species: zebrafish    Sample:

Fig. 6 LDF promote melanogenesis in juvenile zebrafish. A. Toxicity assays in embryos with drugs administration of LDF (0–120 μg/ml); B. The effect of LDF on melanogenesis with drugs administration of LDF (20–80 μg/ml and 1 μM methoxsalen) for 72 h; C-D. The effect of LDH on melanin contents; E. The effect of LDH on the protein expression of MITF, p-MITF, TYR, TRP1, and TRP2.

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