Phospho-Dab1 (Tyr232) Antibody - #AF3029

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (3)  
产品: 磷酸化 Dab1 (Tyr232) 抗体
货号: AF3029
描述: Rabbit polyclonal antibody to Phospho-Dab1 (Tyr232)
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 60kDa; 64kD(Calculated).
蛋白号: O75553
RRID: AB_2834459

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
Phospho-Dab1 (Tyr232) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyrosine 232.
RRID:
AB_2834459
引用格式: Affinity Biosciences Cat# AF3029, RRID:AB_2834459.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

抗原和靶标

免疫原:
Uniprot:

O75553(DAB1_HUMAN) >>Visit HPA database.

基因/基因ID:
DAB1(1600)
表达:
O75553 DAB1_HUMAN:

Mainly expressed in brain.

描述:
DAB1 an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer.
序列:
MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75553 作为底物

Site PTM Type Enzyme
K43 Ubiquitination
K45 Ubiquitination
K84 Sumoylation
Y198 Phosphorylation
Y220 Phosphorylation
Y232 Phosphorylation
S433 Phosphorylation
T506 Phosphorylation
S524 Phosphorylation P24941 (CDK2)
S526 Phosphorylation
S548 Phosphorylation

研究背景

功能:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

翻译修饰:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

组织特异性:

Mainly expressed in brain.

亚基结构:

Associates with the SH2 domains of SRC, FYN and ABL (By similarity). Interacts (phosphorylated on tyrosine residues) with CRK and CRKL (via respective SH2 domain) (By similarity). Interacts with SIAH1, LRP8 and VLDLR (By similarity). Interacts with LRP1. Interacts with APLP1 (via NPXY motif) (By similarity). Interacts with DAB2IP (By similarity).

蛋白家族:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

文献引用

1). Ephrin‑b3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpine‑induced model of epilepsy. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018 (PubMed: 29512697) [IF=5.4]

Application: WB    Species: rat    Sample: hippocampus

Figure 3. |Reelin pathway expression in the hippocampus of rats during the development of SE.(E) Western blot analysis for p‑Dab1. β‑actin was used as an endogenous control. (F) Quantification of western blot results of p‑Dab1, presented as the p‑Dab1/Dab1 ratio. Data are presented as the mean ± standard deviation. ***P<0.0001, **P<0.001 by two‑way analysis of variance with Tukey's post hoc test (n=3 rats/per group). Ep, epilepsy; Con, control; Dab1, disabled 1; p‑Dab, phosphorylated Dab1.

Application: IHC    Species: rat    Sample: hippocampus

Figure 5. |Effect of EphB3‑Fc on reelin pathway expression in the hippocampus post‑SE.(C) Representative immunohistochemical staining of p‑Dab1 following EphB3‑Fc/Fc‑control treatment. Left: representative immunohistochemical staining of p‑Dab1 (scale bar=50 µm); right: high‑magnification bright‑field images and the arrows indicate the immunopositive cells (scale bar=10 µm).

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.