Phospho-ICK (Tyr159) Antibody - #DF8739

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产品: 磷酸化 ICK (Tyr159) 抗体
货号: DF8739
描述: Rabbit polyclonal antibody to Phospho-ICK (Tyr159)
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 71 kDa; 71kD(Calculated).
蛋白号: Q9UPZ9
RRID: AB_2841943

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-ICK (Tyr159) Antibody detects endogenous levels of ICK only when phosphorylated at Tyr159.
RRID:
AB_2841943
引用格式: Affinity Biosciences Cat# DF8739, RRID:AB_2841943.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2210420N10Rik; AI848300; ECO; Heart serine threonine protein kinase; hICK; Intestinal cell (MAK like) kinase; Intestinal cell kinase; KIAA0936; Laryngeal cancer kinase 2; LCK2; MAK related kinase; MGC46090; mICK; MRK; OTTMUSP00000041856; OTTMUSP00000041857; Serine/threonine protein kinase ICK;

抗原和靶标

免疫原:
Uniprot:

Q9UPZ9(CILK1_HUMAN) >>Visit HPA database.

基因/基因ID:
ICK(22858)
表达:
Q9UPZ9 CILK1_HUMAN:

Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.

序列:
MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALRYPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKPKSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYASRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UPZ9 作为底物

Site PTM Type Enzyme
T6 Phosphorylation
T14 Phosphorylation
Y15 Phosphorylation
S17 Phosphorylation
S23 Phosphorylation
S53 Phosphorylation
K56 Ubiquitination
Y156 Phosphorylation
T157 Phosphorylation P50613 (CDK7) , Q8IZL9 (CDK20)
Y159 Phosphorylation Q9UPZ9 (ICK)
Y177 Phosphorylation
T248 Phosphorylation
S254 Phosphorylation
T463 Phosphorylation
T478 Phosphorylation
K491 Methylation
S520 Phosphorylation
S528 Phosphorylation
S529 Phosphorylation
K562 Acetylation
Y583 Phosphorylation
S585 Phosphorylation

翻译修饰 - Q9UPZ9 作为激酶

Substrate Site Source
P46379 (BAG6) T1080 Uniprot
Q8N122 (RPTOR) T908 Uniprot
Q9UPZ9 (ICK) Y159 Uniprot

研究背景

功能:

Required for ciliogenesis. Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).

翻译修饰:

Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity.

细胞定位:

Nucleus. Cytoplasm>Cytosol. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Also found at the ciliary tip (PubMed:24797473). Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360, PubMed:19185282).

Cytoplasm.
Note: Predominant cytoplasmic localization has been observed with a N-terminally GFP-tagged construct.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

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