产品: 磷酸化 OSR1 (Thr185) 抗体
货号: DF8742
描述: Rabbit polyclonal antibody to Phospho-OSR1 (Thr185)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 58 kDa; 58kD(Calculated).
蛋白号: O95747
RRID: AB_2841946

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   规格 价格 库存
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-OSR1 (Thr185) Antibody detects endogenous levels of OSR1 only when phosphorylated at Thr185.
RRID:
AB_2841946
引用格式: Affinity Biosciences Cat# DF8742, RRID:AB_2841946.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2210022N24Rik; 2810422B09Rik; AI462649; AW209236; KIAA1101; mKIAA1101; OSR 1; OSR1; OTTHUMP00000209062; Oxidative stress responsive 1; Oxidative stress responsive 1 protein; Oxidative stress-responsive 1 protein; OXSR 1; OXSR1; OXSR1_HUMAN; Serine/threonine protein kinase OSR1; Serine/threonine-protein kinase OSR1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O95747 OXSR1_HUMAN:

Ubiquitously expressed in all tissue examined.

序列:
MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95747 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K42 Acetylation
K46 Acetylation
K52 Ubiquitination
K105 Ubiquitination
K110 Ubiquitination
K114 Ubiquitination
K139 Ubiquitination
K184 Ubiquitination
T185 Phosphorylation Q9H4A3 (WNK1)
T189 Phosphorylation
K254 Ubiquitination
S263 Phosphorylation
S269 Phosphorylation
K278 Ubiquitination
R287 Methylation
K297 Methylation
K303 Ubiquitination
T310 Phosphorylation
S312 Phosphorylation
S324 Phosphorylation
S325 Phosphorylation Q9H4A3 (WNK1)
T331 Phosphorylation
S339 Phosphorylation P42345 (MTOR)
S347 Phosphorylation
S355 Phosphorylation
S359 Phosphorylation
S369 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
T430 Phosphorylation
S435 Phosphorylation
S443 Phosphorylation
K444 Methylation
R451 Methylation
S510 Phosphorylation

翻译修饰 - O95747 作为激酶

Substrate Site Source
P55011 (SLC12A2) T203 Uniprot
P55011 (SLC12A2) T207 Uniprot
P55011 (SLC12A2) T212 Uniprot
P55011 (SLC12A2) T217 Uniprot
P55011 (SLC12A2) T230 Uniprot
P55017 (SLC12A3) T46 Uniprot
P55017 (SLC12A3) T55 Uniprot
P55017 (SLC12A3) T60 Uniprot
P55017 (SLC12A3) S73 Uniprot
Q13153 (PAK1) T84 Uniprot
Q13621 (SLC12A1) T95 Uniprot
Q13621 (SLC12A1) T100 Uniprot
Q13621 (SLC12A1) T105 Uniprot
Q13621 (SLC12A1) T118 Uniprot
Q13621 (SLC12A1) S120 Uniprot

研究背景

功能:

Phosphorylates RELL1, RELL2 and RELT. Phosphorylates PAK1. Phosphorylates PLSCR1 in the presence of RELT.

翻译修饰:

Autophosphorylated.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed in all tissue examined.

亚基结构:

Interacts with PAK1. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress (By similarity). Interacts with RELL1, RELL2 and RELT. Interacts with PLSCR1 in the presence of RELT.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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