Phospho-ELK1 (Ser383) Antibody - #AF3212
(2)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3212, RRID:AB_2834504.
展开/折叠
ELK 1; Elk1; ELK1 member of ETS oncogene family; ELK1 protein; ELK1, ETS transcription factor; ELK1_HUMAN; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene;
抗原和靶标
- P19419 ELK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPSVTLWQFLLQLLREQGNGHIISWTSRDGGEFKLVDAEEVARLWGLRKNKTNMNYDKLSRALRYYYDKNIIRKVSGQKFVYKFVSYPEVAGCSTEDCPPQPEVSVTSTMPNVAPAAIHAAPGDTVSGKPGTPKGAGMAGPGGLARSSRNEYMRSGLYSTFTIQSLQPQPPPHPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEELEVAGERGFVPETTKAEPEVPPQEGVPARLPAVVMDTAGQAGGHAASSPEISQPQKGRKPRDLELPLSPSLLGGPGPERTPGSGSGSGLQAPGPALTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGSAQVHIPSISVDGLSTPVVLSPGPQKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19419 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y57 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| K230 | Sumoylation | Uniprot | |
| K249 | Sumoylation | Uniprot | |
| K254 | Sumoylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S326 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T353 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14) , P45983 (MAPK8) , Q9UL54 (TAOK2) , O75582 (RPS6KA5) | Uniprot |
| S389 | Phosphorylation | Q16539 (MAPK14) , P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , P45984 (MAPK9) | Uniprot |
| S416 | Phosphorylation | Uniprot | |
| T417 | Phosphorylation | Q13523 (PRPF4B) | Uniprot |
| S422 | Phosphorylation | P27361 (MAPK3) | Uniprot |
研究背景
Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).
Nucleus.
Lung and testis.
Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9. Interacts with POU1F1.
Belongs to the ETS family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
文献引用
Application: WB Species: Human Sample: gastric cancer tissues
限制条款
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