| 产品: | 磷酸化 ATP1A1 (Ser23) 抗体 |
| 货号: | AF3109 |
| 描述: | Rabbit polyclonal antibody to Phospho-ATP1A1 (Ser23) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 113kDa; 113kD(Calculated). |
| 蛋白号: | P05023 |
| RRID: | AB_2834546 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3109, RRID:AB_2834546.
展开/折叠
A1A1; AT1A1; AT1A1_HUMAN; ATP1A1; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting subunit alpha 1; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+; K+ ATPase alpha subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na;K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1;
抗原和靶标
- P05023 AT1A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGFSMLLWIGAILCFLAYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVDWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY
翻译修饰 - P05023 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| Y10 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Q05513 (PRKCZ) , P28482 (MAPK1) | Uniprot |
| K21 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| R61 | Methylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| R198 | Methylation | Uniprot | |
| R204 | Methylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| C249 | S-Nitrosylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| R264 | Methylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| C459 | S-Nitrosylation | Uniprot | |
| C463 | S-Nitrosylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| K487 | Acetylation | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| Y488 | Phosphorylation | Uniprot | |
| K494 | Methylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| R517 | Methylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| K526 | Sumoylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| Y542 | Phosphorylation | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| T623 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| T640 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| K661 | Ubiquitination | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| Y684 | Phosphorylation | Uniprot | |
| T686 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| C705 | S-Nitrosylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K833 | Ubiquitination | Uniprot | |
| K840 | Ubiquitination | Uniprot | |
| K843 | Ubiquitination | Uniprot | |
| Y902 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K950 | Ubiquitination | Uniprot | |
| K952 | Ubiquitination | Uniprot | |
| K1019 | Ubiquitination | Uniprot |
研究背景
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
Cell membrane>Sarcolemma>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3. Interacts with SIK1 (By similarity). Binds the HLA class II histocompatibility antigen DR1. Interacts with SLC35G1 and STIM1.
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
研究领域
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.
· Organismal Systems > Digestive system > Protein digestion and absorption.
· Organismal Systems > Digestive system > Mineral absorption.
限制条款
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