Phospho-hnRNP C1/C2 (Ser260) Antibody - #AF3118

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产品: 磷酸化 hnRNP C1/C2 (Ser260) 抗体
货号: AF3118
描述: Rabbit polyclonal antibody to Phospho-hnRNP C1/C2 (Ser260)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Rabbit, Dog, Xenopus
分子量: 41kDa; 34kD(Calculated).
蛋白号: P07910
RRID: AB_2834553

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-hnRNP C1/C2 (Ser260) Antibody detects endogenous levels of hnRNP C1/C2 only when phosphorylated at Serine 260.
RRID:
AB_2834553
引用格式: Affinity Biosciences Cat# AF3118, RRID:AB_2834553.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C1; C2; Heterogeneous nuclear ribonucleoprotein C (C1/C2); Heterogeneous nuclear ribonucleoprotein C; Heterogeneous nuclear ribonucleoproteins C1/C2; HNRNP; hnRNP C1 / hnRNP C2; hnRNP C1/C2; Hnrnpc; HNRPC; HNRPC_HUMAN; MGC104306; MGC105117; MGC117353; MGC131677; Nuclear ribonucleoprotein particle C1 protein; Nuclear ribonucleoprotein particle C2 protein; SNRPC;

抗原和靶标

免疫原:
Uniprot:

P07910(HNRPC_HUMAN) >>Visit HPA database.

基因/基因ID:
HNRNPC(3183)
描述:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
序列:
MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRSAAEMYGSVTEHPSPSPLLSSSFDLDYDFQRDYYDRMYSYPARVPPPPPIARAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDLQAIKKELTQIKQKVDSLLENLEKIEKEQSKQAVEMKNDKSEEEQSSSSVKKDETNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEGEDDRDSANGEDDS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Pig
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07910 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
K8 Acetylation
K8 Sumoylation
K8 Ubiquitination
T9 Phosphorylation
K29 Ubiquitination
K30 Ubiquitination
S31 Phosphorylation
S38 Phosphorylation
K39 Acetylation
K39 Methylation
K39 Ubiquitination
K42 Sumoylation
K42 Ubiquitination
S47 Phosphorylation
K50 Acetylation
K50 Sumoylation
K50 Ubiquitination
Y57 Phosphorylation
R61 Methylation
K89 Sumoylation
K89 Ubiquitination
K98 Methylation
S100 Phosphorylation
Y105 Phosphorylation
S107 Phosphorylation
T109 Phosphorylation
S113 Phosphorylation
S115 Phosphorylation
S119 Phosphorylation
S120 Phosphorylation
S121 Phosphorylation
Y126 Phosphorylation
Y132 Phosphorylation
Y133 Phosphorylation
R135 Methylation
Y137 Phosphorylation
S138 Phosphorylation
Y139 Phosphorylation
R142 Methylation
R151 Methylation
S156 Phosphorylation
K157 Acetylation
R160 Methylation
S162 Phosphorylation
T165 Phosphorylation
S166 Phosphorylation
K170 Acetylation
K170 Ubiquitination
S171 Phosphorylation
S175 Phosphorylation
K176 Sumoylation
K176 Ubiquitination
K187 Acetylation
K187 Ubiquitination
K189 Methylation
K189 Ubiquitination
K197 Ubiquitination
K198 Ubiquitination
K204 Ubiquitination
K206 Ubiquitination
S209 Phosphorylation
K216 Acetylation
K216 Ubiquitination
K219 Ubiquitination
K223 Sumoylation
K223 Ubiquitination
K229 Sumoylation
K229 Ubiquitination
K232 Acetylation
K232 Sumoylation
K232 Ubiquitination
S233 Phosphorylation
S238 Phosphorylation
S239 Phosphorylation
S240 Phosphorylation
S241 Phosphorylation
K243 Sumoylation
K243 Ubiquitination
K244 Ubiquitination
T247 Phosphorylation
K250 Sumoylation
K250 Ubiquitination
S253 Phosphorylation P48729 (CSNK1A1)
S260 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
K284 Ubiquitination
S299 Phosphorylation P48729 (CSNK1A1)
S306 Phosphorylation

研究背景

功能:

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.

翻译修饰:

Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.

Sumoylated. Sumoylation reduces affinity for mRNA.

细胞定位:

Nucleus.
Note: Component of ribonucleosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with DHX9; this interaction is direct, enhanced probably by their concomitant binding to RNA and mediates the attachment to actin filaments.

蛋白家族:

Belongs to the RRM HNRPC family. RALY subfamily.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

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