产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3171, RRID:AB_2834603.
展开/折叠
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1;
抗原和靶标
- P15056 BRAF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15056 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S76 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S151 | Phosphorylation | P28482 (MAPK1) , P15056 (BRAF) , P27361 (MAPK3) | Uniprot |
| K164 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | P31751 (AKT2) , Q9Y243 (AKT3) , O00141 (SGK1) | Uniprot |
| S365 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) , Q9Y243 (AKT3) | Uniprot |
| T373 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S394 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| T401 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S405 | Phosphorylation | Uniprot | |
| K418 | Acetylation | Uniprot | |
| S419 | Phosphorylation | P15056 (BRAF) | Uniprot |
| S428 | Phosphorylation | Q9Y243 (AKT3) , P31751 (AKT2) | Uniprot |
| S429 | Phosphorylation | Q9Y243 (AKT3) , P31749 (AKT1) | Uniprot |
| S430 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S431 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | P31751 (AKT2) | Uniprot |
| S445 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Q13153 (PAK1) , Q15382 (RHEB) | Uniprot |
| S447 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| Y566 | Phosphorylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | P15056 (BRAF) | Uniprot |
| R671 | Methylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| K687 | Ubiquitination | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S732 | Phosphorylation | Uniprot | |
| Y746 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | P28482 (MAPK1) , P15056 (BRAF) | Uniprot |
| T753 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y760 | Phosphorylation | Uniprot |
翻译修饰 - P15056 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P15056 (BRAF) | S147 | Uniprot |
| P15056 (BRAF) | S151 | Uniprot |
| P15056 (BRAF) | T373 | Uniprot |
| P15056 (BRAF) | S419 | Uniprot |
| P15056 (BRAF) | S614 | Uniprot |
| P15056 (BRAF) | S750 | Uniprot |
| P33981 (TTK) | S281 | Uniprot |
| P33981 (TTK) | S436 | Uniprot |
| P36507 (MAP2K2) | S222 | Uniprot |
| P36507 (MAP2K2) | S226 | Uniprot |
| P68104 (EEF1A1) | S21 | Uniprot |
| P68104 (EEF1A1) | T88 | Uniprot |
| Q02750 (MAP2K1) | S218 | Uniprot |
| Q02750-1 (MAP2K1) | S222 | Uniprot |
| Q05639 (EEF1A2) | S21 | Uniprot |
| Q92934 (BAD) | S75 | Uniprot |
| Q92934 (BAD) | S99 | Uniprot |
| Q92934 (BAD) | S118 | Uniprot |
| Q92934 (BAD) | S134 | Uniprot |
研究背景
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.
Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
Nucleus. Cytoplasm. Cell membrane.
Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Brain and testis.
Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Heterodimerizes (via N-terminus) with KSR1 (via N-terminus) or KSR2 (via N-terminus) in a MAP2K1-dependent manner. Interacts with MAP2K1 and MAP2K2. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2. Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, MAP2K1 and KSR1. Interacts with FNIP1 and FNIP2.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
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