产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3182, RRID:AB_2834614.
展开/折叠
Oncogene vav; p95Vav; Proto-oncogene vav; Protooncogene vav; VAV 1; VAV 1 oncogene; VAV; Vav proto oncogene; VAV_HUMAN; VAV1;
抗原和靶标
- P15498 VAV_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELWRQCTHWLIQCRVLPPSHRVTWDGAQVCELAQALRDGVLLCQLLNNLLPHAINLREVNLRPQMSQFLCLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNRGIMPFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDLMRSEPVSMPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKYKERFLVYGRYCSQVESASKHLDRVAAAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLAHYGRPKIDGELKITSVERRSKMDRYAFLLDKALLICKRRGDSYDLKDFVNLHSFQVRDDSSGDRDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15498 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K73 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | P06239 (LCK) | Uniprot |
| Y160 | Phosphorylation | P06239 (LCK) | Uniprot |
| Y174 | Phosphorylation | P43405 (SYK) , P06239 (LCK) | Uniprot |
| S180 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| K194 | Acetylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K252 | Acetylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| Y267 | Phosphorylation | P00519 (ABL1) | Uniprot |
| Y280 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K345 | Acetylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| K487 | Acetylation | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| K588 | Ubiquitination | Uniprot | |
| Y603 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| K716 | Acetylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| K732 | Acetylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| K733 | Acetylation | Uniprot | |
| Y745 | Phosphorylation | Uniprot | |
| K751 | Ubiquitination | Uniprot | |
| K755 | Ubiquitination | Uniprot | |
| K766 | Ubiquitination | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| Y783 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| Y791 | Phosphorylation | Uniprot | |
| K804 | Ubiquitination | Uniprot | |
| K810 | Ubiquitination | Uniprot | |
| K815 | Ubiquitination | Uniprot | |
| Y826 | Phosphorylation | Uniprot | |
| Y836 | Phosphorylation | Uniprot | |
| Y841 | Phosphorylation | Uniprot | |
| S842 | Phosphorylation | Uniprot | |
| Y844 | Phosphorylation | Uniprot |
研究背景
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS) (By similarity). Phosphorylated by FYN.
Widely expressed in hematopoietic cells but not in other cell types.
Interacts with SHB. Interacts with APS, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. May interact with CCPG1. Interacts with CLNK. Interacts with THEMIS2 (By similarity). Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2 (By similarity). Interacts with HCK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54. Interacts with CD6 (By similarity).
The DH domain is involved in interaction with CCPG1.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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