Phospho-CREB (Ser133) Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	磷酸化 CREB (Ser133) 抗体
货号:	AF3189
描述:	Rabbit polyclonal antibody to Phospho-CREB (Ser133)
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	43kDa; 37kD(Calculated).
蛋白号:	P16220
RRID:	AB_2834621

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1300	现货
100ul	RMB¥ 2400	现货
200ul	RMB¥ 3200	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.

RRID:

AB_2834621
引用格式: Affinity Biosciences Cat# AF3189, RRID:AB_2834621.

偶联:

Unconjugated.

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Active transcription factor CREB; cAMP response element binding protein 1; cAMP response element binding protein; cAMP responsive element binding protein 1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; CREB1_HUMAN; Cyclic AMP-responsive element-binding protein 1; MGC9284; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; Transactivator protein;

抗原和靶标

免疫原:

Uniprot:

P16220(CREB1_HUMAN) >>Visit HPA database.

基因/基因ID:

CREB1(1385)

描述:

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.

序列:

P16220 CREB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P16220 作为底物

P16220

Site	PTM Type	Enzyme	Source
S40	Phosphorylation		Uniprot
S80	Phosphorylation		Uniprot
S98	Phosphorylation	Q15139 (PRKD1)	Uniprot
T100	Phosphorylation	Q13315 (ATM)	Uniprot
S108	Phosphorylation	P68400 (CSNK2A1) , P48729 (CSNK1A1)	Uniprot
S111	Phosphorylation	P68400 (CSNK2A1) , P48729 (CSNK1A1) , Q13315 (ATM)	Uniprot
S114	Phosphorylation	P48729 (CSNK1A1)	Uniprot
S117	Phosphorylation		Uniprot
T119	Phosphorylation		Uniprot
S121	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
S129	Phosphorylation	P49841 (GSK3B)	Uniprot
S133	Phosphorylation	Q16539 (MAPK14) , P31751 (AKT2) , Q02156 (PRKCE) , O43781 (DYRK3) , Q96NX5 (CAMK1G) , Q16566 (CAMK4) , P17612 (PRKACA) , Q6SA08 (TSSK4) , Q99986 (VRK1) , Q13557 (CAMK2D) , Q15418 (RPS6KA1) , O75676 (RPS6KA4) , P28482 (MAPK1) , Q14012 (CAMK1) , Q06187 (BTK) , P51812 (RPS6KA3) , Q13627 (DYRK1A) , O75582 (RPS6KA5) , P41279 (MAP3K8) , O00141 (SGK1) , P27361 (MAPK3) , P31749 (AKT1) , Q13315 (ATM) , Q15349 (RPS6KA2) , Q9Y243 (AKT3) , P49137 (MAPKAPK2) , Q15139 (PRKD1)	Uniprot
Y134	Phosphorylation		Uniprot
K136	Acetylation		Uniprot
K136	Ubiquitination		Uniprot
S142	Phosphorylation	Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)	Uniprot
S143	Phosphorylation		Uniprot
S156	Phosphorylation	P48729 (CSNK1A1)	Uniprot
T256	Phosphorylation		Uniprot
S270	Phosphorylation	P06493 (CDK1)	Uniprot
S271	Phosphorylation	Q9H2X6 (HIPK2) , P06493 (CDK1)	Uniprot
K285	Sumoylation		Uniprot
K304	Sumoylation		Uniprot
K309	Ubiquitination		Uniprot
R314	Methylation		Uniprot
K323	Ubiquitination		Uniprot
K330	Ubiquitination		Uniprot
K333	Ubiquitination		Uniprot
K339	Ubiquitination		Uniprot
S340	Phosphorylation		Uniprot

研究背景

P16220_CREB1_HUMAN

功能:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

翻译修饰:

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-133.

Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

细胞定位:

Nucleus.

亚基结构:

Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-133. Forms a complex with KMT2A and CREBBP.

(Microbial infection) Interacts with hepatitis B virus/HBV protein X.

(Microbial infection) Interacts with HTLV-1 protein Tax.

蛋白家族:

Belongs to the bZIP family.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

文献引用

1). β-asarone improves cognitive impairment and alleviates autophagy in mice with vascular dementia via the cAMP/PKA/CREB pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38039902) [IF=7.9]

2). Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics. PHYTOMEDICINE, 2021 (PubMed: 33360346) [IF=7.9]

Application: WB Species: mice Sample: hippocampus

Fig. 11. Effects of URE on 5-HT1A signaling pathway. (A) Western blotting analysis of 5-HT1A, BDNF, p-CREB, CREB, p-PKA, and PKA in hippocampus. (B-E) Quantitative data of 5-HT1A (B), BDNF (C), p-CREB/CREB (D), and p-PKA/PKA (E), data represent mean ± SEM (n = 3), ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the UCMS group. (F) The agonistic effect of URE against 5-HT1A receptor.

3). The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine, 2021 (PubMed: 34560247) [IF=7.4]

4). Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. Phytotherapy Research, 2023 (PubMed: 36428266) [IF=7.2]

5). MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021 (PubMed: 34425535) [IF=6.8]

Application: WB Species: Rat Sample: NG108–15 cells

Fig. 4. mRNA and protein expression of Dusp2, p38 MAPK, CREB and JNK in NG108–15 cells treated with F, Al and FA. (A/B) The graphs show the results of the statistical analysis of Dusp2, p38 MAPK, CREB and JNK mRNA expression levels based on qRT-PCR. (C/D/E/F/G/H/I) The graphs show signaling pathway protein expression in NG108–15 cells as assessed by Western blotting analysis. a P < 0.05, compared with the control group; b P < 0.05, compared with the F group; c P < 0.05, compared with the Al group (n = 6).

6). A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ. Journal of Ginseng Research, 2022 (PubMed: 36090685) [IF=6.3]

Application: WB Species: Rat Sample:

Fig. 5 Effects of fluoxetine (FLU) or PPD on the immobility time in the forced swimming test (FST) (A) and tail suspension test (TST) (B) in the corticosterone (CORT)-induced depression model. Representative Western blot images of GSK 3β, p-Ser9 GSK 3β, CREB, p-Ser133 CREB, BDNF, and β-actin expression levels (C). Western blot analysis showing relative protein levels of p-Ser133 CREB (D), p-Ser9 GSK 3β (E), and BDNF (F). ∗p < 0.05 vs. the control group, nsno statistical significance vs. the control group, #p < 0.05 vs. the CORT group.

7). Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function, 2022 (PubMed: 35899807) [IF=6.1]

8). Combined effects of di (2-ethylhexyl) phthalate and bisphenol A on thyroid hormone homeostasis in adolescent female rats. Environmental science and pollution research international, 2020 (PubMed: 32681327) [IF=5.8]

Application: IHC Species: rat Sample: thyroid

Fig. 5| Effects of DEHP and BPA on relative gene expression in the thyroid (n = 7). a Immunohistochemistry for Esr1 in the thyroid(original magnification, × 20). Scale bar = 200 μm. b The average optical density of Esr1 in the thyroid. c The mRNA expression of Esr1.d Immunohistochemistry for phosphorylation of Creb in the thyroid(original magnification, × 20).

9). Purple sweet potato delphinidin-3-rutin represses glioma proliferation by inducing miR-20b-5p/Atg7-dependent cytostatic autophagy. Molecular Therapy - Oncolytics, 2022 (PubMed: 36090477) [IF=5.7]

Application: WB Species: Human Sample: U251 and A172 cells

Figure 7 PSPD3R induces autophagy in glioma cells through the Akt/Creb signaling pathway (A) U251 and A172 cells were treated with the indicated concentrations of PSPD3R for 24 h. Atg7, phosphorylation of Creb and Akt, and LC3 expression were measured by immunoblotting. (B) U251 and A172 cells were transfected with a constitutively active Akt plasmid (CA-Akt) for 24 h and were then treated with 50 μM PSPD3R for another 24 h. Cell lysates were collected and performed for immunoblotting of Akt phosphorylation, Creb phosphorylation, and LC3 lipidation. (C) U251 and A172 cells were transfected with overexpression-Creb plasmid in the presence or absence of PSPD3R (50 μM) for 24 h, and then cell lysates were collected and performed for immunoblotting of Creb and LC3 lipidation. (D) U251 and A172 cells were transfected with Creb shRNA plasmids (sh-Creb), and then cell lysates were collected and performed for immunoblotting of Creb and LC3 lipidation. (E) U251 and A172 cells were transfected with overexpression-Creb plasmid in the presence or absence of PSPD3R (50 μM) for 24 h, and then cells stained with acridine orange. Autophagosome formation of glioma cells were observed by confocal microscope. Scale bar, 10 µm. (F) U251 and A172 cells were transfected with sh-Creb plasmid or shNC, then the expression of Atg7 mRNA was measured by quantitative real-time PCR. Data are presented as mean ± SD from three independent experiments. t test for two experimental groups comparisons

10). Metformin Modulates High Glucose-Incubated Human Umbilical Vein Endothelial Cells Proliferation and Apoptosis Through AMPK/CREB/BDNF Pathway. Frontiers in Pharmacology, 2018 (PubMed: 30459620) [IF=5.6]

Application: WB Species: human Sample: HUVECs

FIGURE 5 | BDNF expression was regulated by metformin through CREB activation. (A) Western blot analysis of CREB and pCREB expression in HUVECs treated with NG, HG (33.3 mmol/l), and HG + MET (0.01 mmol/l) conditions. Histograms represent the relative pCREB/CREB intensity.

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Phospho-CREB (Ser133) Antibody - #AF3189

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