| 产品: | 磷酸化 CKB (Ser199) 抗体 |
| 货号: | AF8602 |
| 描述: | Rabbit polyclonal antibody to Phospho-CKB (Ser199) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 43 kDa; 43kD(Calculated). |
| 蛋白号: | P12277 |
| RRID: | AB_2843453 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8602, RRID:AB_2843453.
展开/折叠
B CK; B-CK; BB-CK; BCK; Brain creatine kinase; Ckb; CKBB; Creatine kinase B; Creatine kinase B chain; Creatine kinase B type; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine kinase brain type; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211; HEL S 29; KCRB_HUMAN;
抗原和靶标
- P12277 KCRB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12277 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| R13 | Methylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| R43 | Methylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| R96 | Methylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| R138 | Methylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| R172 | Methylation | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| Y174 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| R215 | Methylation | Uniprot | |
| K223 | Sumoylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K267 | Acetylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K307 | Sumoylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| K313 | Acetylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| R320 | Methylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot |
研究背景
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Cytoplasm.
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
Belongs to the ATP:guanido phosphotransferase family.
研究领域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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