Phospho-PFKP (Tyr52) Antibody - #AF8610

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产品: 磷酸化 PFKP (Tyr52) 抗体
货号: AF8610
描述: Rabbit polyclonal antibody to Phospho-PFKP (Tyr52)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 84 KD; 86kD(Calculated).
蛋白号: Q01813
RRID: AB_2843460

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:100-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-PFKP (Tyr52) Antibody detects endogenous levels of PFKP only when phosphorylated at Y52.
RRID:
AB_2843460
引用格式: Affinity Biosciences Cat# AF8610, RRID:AB_2843460.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1200015H23Rik; 6 phosphofructokinase type C; 6 phosphofructokinase, platelet type; 6-phosphofructokinase; 6-phosphofructokinase type C; 9330125N24Rik; FLJ40226; K6PP; K6PP_HUMAN; MGC105718; PFK C; PFK, fibroblast type; PFK-C; PFKF; PFKP; Phosphofructo 1 kinase isozyme C; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; Phosphofructokinase platelet; Phosphohexokinase; platelet type;

抗原和靶标

免疫原:
Uniprot:

Q01813(PFKAP_HUMAN) >>Visit HPA database.

基因/基因ID:
PFKP(5214)
描述:
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
序列:
MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q01813 作为底物

Site PTM Type Enzyme
M1 Acetylation
S6 Phosphorylation
K10 Ubiquitination
S12 Phosphorylation
K15 Ubiquitination
S21 Phosphorylation
K25 Ubiquitination
Y52 Phosphorylation
Y64 Phosphorylation
K109 Acetylation
K109 Methylation
K109 Ubiquitination
K139 Ubiquitination
S142 Phosphorylation
K156 Acetylation
K156 Ubiquitination
T211 Phosphorylation
K287 Ubiquitination
K289 Ubiquitination
R310 Methylation
T313 Phosphorylation
R319 Methylation
S386 Phosphorylation
T393 Phosphorylation
K395 Acetylation
K395 Ubiquitination
K400 Ubiquitination
C411 S-Nitrosylation
Y447 Phosphorylation
K455 Ubiquitination
K459 Ubiquitination
K480 Acetylation
K480 Ubiquitination
K486 Acetylation
K486 Ubiquitination
Y487 Phosphorylation
K567 Ubiquitination
K574 Acetylation
Y586 Phosphorylation
K625 Ubiquitination
K627 Ubiquitination
Y645 Phosphorylation
Y651 Phosphorylation
Y654 Phosphorylation
K666 Ubiquitination
K688 Acetylation
K688 Ubiquitination
K700 Acetylation
K724 Ubiquitination
K736 Acetylation
K736 Methylation
K736 Ubiquitination
K737 Methylation
K737 Ubiquitination
K747 Acetylation
K747 Ubiquitination
K753 Ubiquitination
K759 Ubiquitination
K765 Ubiquitination
S783 Phosphorylation

研究背景

功能:

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

翻译修饰:

GlcNAcylation decreases enzyme activity.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). In platelets, brain and fibroblasts, PFK contains a higher proportion of PFKP subunits. The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable).

蛋白家族:

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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