Phospho-KLF5 (Ser303) Antibody - #AF7042

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产品: 磷酸化 KLF5 (Ser303) 抗体
货号: AF7042
描述: Rabbit polyclonal antibody to Phospho-KLF5 (Ser303)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 51kDa; 51kD(Calculated).
蛋白号: Q13887
RRID: AB_2843482

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-KLF5 (Ser303) Antibody detects endogenous levels of KLF5 only when phosphorylated at Ser303.
RRID:
AB_2843482
引用格式: Affinity Biosciences Cat# AF7042, RRID:AB_2843482.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2;

抗原和靶标

免疫原:
Uniprot:

Q13887(KLF5_HUMAN) >>Visit HPA database.

基因/基因ID:
KLF5(688)
表达:
Q13887 KLF5_HUMAN:

Expressed only in testis and placenta.

序列:
MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVNSQNIQPVRYNRRSNPDLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13887 作为底物

Site PTM Type Enzyme
K31 Ubiquitination
K110 Sumoylation
K110 Ubiquitination
S116 Phosphorylation
S153 Phosphorylation Q05655 (PRKCD)
K162 Sumoylation
K209 Sumoylation
S303 Phosphorylation P49841 (GSK3B)
T323 Phosphorylation
T346 Phosphorylation
Y359 Phosphorylation
S363 Phosphorylation
K369 Acetylation
Y377 Phosphorylation
K391 Sumoylation
K391 Ubiquitination
T396 Phosphorylation
T398 Phosphorylation
K401 Ubiquitination
S441 Phosphorylation
S443 Phosphorylation

研究背景

功能:

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

翻译修饰:

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed only in testis and placenta.

亚基结构:

Interacts with WWP1.

蛋白家族:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

文献引用

1). Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop. Cell death & disease, 2022 (PubMed: 36042208) [IF=9.0]

Application: WB    Species: Human    Sample: MDA-MB-231 cells

Fig. 4 DCC-2036 and AXL regulate the KLF5 protein level through Akt/GSK3β pathway. A DCC-2036 decreased the phosphorylation of AKT (Ser473), GSK3β (Ser9), and total KLF5, and increased the phosphorylation of KLF5 (Ser303). TNBC cells were treated with DCC-2036 for 48 h, followed by the WB assay. B Gas6 promoted the increase of p-AKT, p-GSK3β, and total KLF5, as well as the decrease of p-KLF5 in MDA-MB-231 cells and HS-578T cells. Cells were treated with Gas6 (200 ng/ml) for 0, 30, 60, 90, and 120 min, and then examined by WB.

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