产品: 磷酸化 SLC16A1 (Ser213) 抗体
货号: AF7044
描述: Rabbit polyclonal antibody to Phospho-SLC16A1 (Ser213)
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 54kDa; 54kD(Calculated).
蛋白号: P53985
RRID: AB_2843484

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-SLC16A1 (Ser213) Antibody detects endogenous levels of SLC16A1 only when phosphorylated at Ser213.
RRID:
AB_2843484
引用格式: Affinity Biosciences Cat# AF7044, RRID:AB_2843484.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ36745; HHF7; MCT 1; MCT; MGC44475; Monocarboxylate transporter 1; Monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; MOT1_HUMAN; Slc16a1; SLC16A1 protein; Solute carrier family 16 (monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1 (monocarboxylic acid transporter 1); Solute carrier family 16 member 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P53985 MOT1_HUMAN:

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.

序列:
MPPAVGGPVGYTPPDGGWGWAVVIGAFISIGFSYAFPKSITVFFKEIEGIFHATTSEVSWISSIMLAVMYGGGPISSILVNKYGSRIVMIVGGCLSGCGLIAASFCNTVQQLYVCIGVIGGLGLAFNLNPALTMIGKYFYKRRPLANGLAMAGSPVFLCTLAPLNQVFFGIFGWRGSFLILGGLLLNCCVAGALMRPIGPKPTKAGKDKSKASLEKAGKSGVKKDLHDANTDLIGRHPKQEKRSVFQTINQFLDLTLFTHRGFLLYLSGNVIMFFGLFAPLVFLSSYGKSQHYSSEKSAFLLSILAFVDMVARPSMGLVANTKPIRPRIQYFFAASVVANGVCHMLAPLSTTYVGFCVYAGFFGFAFGWLSSVLFETLMDLVGPQRFSSAVGLVTIVECCPVLLGPPLLGRLNDMYGDYKYTYWACGVVLIISGIYLFIGMGINYRLLAKEQKANEQKKESKEEETSIDVAGKPNEVTKAAESPDQKDTDGGPKEEESPV

翻译修饰 - P53985 作为底物

Site PTM Type Enzyme
S33 Phosphorylation
Y34 Phosphorylation
K211 Ubiquitination
S213 Phosphorylation
K216 Ubiquitination
K224 Ubiquitination
K239 Ubiquitination
S244 Phosphorylation
T248 Phosphorylation
K459 Ubiquitination
S461 Phosphorylation
K462 Ubiquitination
T466 Phosphorylation
S467 Phosphorylation
K473 Ubiquitination
K479 Ubiquitination
S483 Phosphorylation
K487 Ubiquitination
T489 Phosphorylation
K494 Methylation
K494 Ubiquitination
S498 Phosphorylation

研究背景

功能:

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.

亚基结构:

Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.

蛋白家族:

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

文献引用

1). Effects of Asiaticoside Treatment on the Survival of Random Skin Flaps in Rats. JOURNAL OF INVESTIGATIVE SURGERY, 2019 (PubMed: 30898065) [IF=1.9]

2). Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35783526)

Application: WB    Species: Rabbit    Sample:

Figure 6 The expression level of TF, p-TF, SLC16A1, and p-SLC16A1 was evaluated by the Western blot assay. Compared to the control group, p-TF/TF was significantly upregulated in the model group. In addition, compared to control, the expression level of p-SLC16A1/SLC16A1 was found significantly suppressed in the model group (p < 0.05).

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