产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7053, RRID:AB_2843493.
展开/折叠
H4 K20 HMTase; H4 K20 specific histone methyltransferase; H4-K20-HMTase SETD8; Histone H4 K20 methyltransferase; Histone lysine N methyltransferase H4 lysine 20 specific; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; N-lysine methyltransferase SETD8; Pr SET 7; Pr SET7; PR-Set7; PR/SET domain containing protein 07; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PrSET7; SET 07; SET 8; SET domain containing (lysine methyltransferase) 8; SET domain containing 8; SET domain containing lysine methyltransferase 8; SET domain containing protein 8; SET domain-containing protein 8; SET07; SET8; SETD 8; setd8; SETD8_HUMAN;
抗原和靶标
- Q9NQR1 KMT5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NQR1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y96 | Phosphorylation | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K151 | Sumoylation | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Sumoylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot |
研究背景
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.
Ubiquitinated and degraded by the DCX(DTL) complex.
Nucleus. Chromosome.
Note: Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Interacts with L3MBTL1. Isoform 2 interacts with SIRT2 (phosphorylated form); the interaction is direct, stimulates KMT5A-mediated methyltransferase activity at histone H4 'Lys-20' (H4K20me1) and is increased in a H(2)O(2)-induced oxidative stress-dependent manner.
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
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