| 产品: | 磷酸化 EIF4A1 (Tyr48) 抗体 |
| 货号: | AF7054 |
| 描述: | Rabbit polyclonal antibody to Phospho-EIF4A1 (Tyr48) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 46kDa; 46kD(Calculated). |
| 蛋白号: | P60842 |
| RRID: | AB_2843494 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7054, RRID:AB_2843494.
展开/折叠
ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN;
抗原和靶标
- P60842 IF4A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P60842 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Methylation | Uniprot | |
| K54 | Sumoylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| C66 | S-Nitrosylation | Uniprot | |
| K68 | Sumoylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R168 | Methylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Methylation | Uniprot | |
| K193 | Sumoylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| K202 | Sumoylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K225 | Sumoylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Sumoylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| R255 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K291 | Sumoylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K309 | Acetylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| R316 | Methylation | Uniprot | |
| R353 | Methylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K381 | Ubiquitination | Uniprot |
研究背景
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.
Belongs to the DEAD box helicase family. eIF4A subfamily.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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