| 产品: | 磷酸化 CDC5L (Tyr232) 抗体 |
| 货号: | AF7063 |
| 描述: | Rabbit polyclonal antibody to Phospho-CDC5L (Tyr232) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 92kDa; 92kD(Calculated). |
| 蛋白号: | Q99459 |
| RRID: | AB_2843503 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7063, RRID:AB_2843503.
展开/折叠
CDC5; CDC5 cell division cycle 5-like (S. pombe); Cdc5 related protein; CDC5-LIKE; Cdc5-like protein; Cdc5l; CDC5L_HUMAN; CEF1; Cell division cycle 5 S. pombe homolog of; Cell division cycle 5-like protein; dJ319D22.1; KIAA0432; PCDC5RP; Pombe Cdc5 related protein; Pombe Cdc5-related protein;
抗原和靶标
- Q99459 CDC5L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99459 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K25 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K165 | Methylation | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| K219 | Sumoylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| R252 | Methylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T411 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T415 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| Y464 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| Y511 | Phosphorylation | Uniprot | |
| Y621 | Phosphorylation | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K626 | Acetylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| T797 | Phosphorylation | Uniprot |
研究背景
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Nucleus. Nucleus speckle. Cytoplasm.
Note: May shuttle between cytoplasm and nucleus.
Ubiquitously expressed in both fetal and adult tissues.
Homodimer. Interacts with DAPK3 (By similarity). Component of the precatalytic, catalytic and postcatalytic spliceosome complexes. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).
Belongs to the CEF1 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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