Phospho-Gli1 (Thr1074) Antibody - #AF7070

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产品: 磷酸化 Gli1 (Thr1074) 抗体
货号: AF7070
描述: Rabbit polyclonal antibody to Phospho-Gli1 (Thr1074)
应用: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 119kDa; 118kD(Calculated).
蛋白号: P08151
RRID: AB_2843510

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(90%), Zebrafish(80%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(90%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-Gli1 (Thr1074) Antibody detects endogenous levels of Gli1 only when phosphorylated at Thr1074.
RRID:
AB_2843510
引用格式: Affinity Biosciences Cat# AF7070, RRID:AB_2843510.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1;

抗原和靶标

免疫原:
Uniprot:

P08151(GLI1_HUMAN) >>Visit HPA database.

基因/基因ID:
GLI1(2735)
表达:
P08151 GLI1_HUMAN:

Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).

序列:
MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Chicken
100
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Zebrafish
80
Rabbit
80
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P08151 作为底物

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T63 Phosphorylation
S69 Phosphorylation
S84 Phosphorylation P23443 (RPS6KB1)
S102 Phosphorylation P54646 (PRKAA2)
S130 Phosphorylation
R161 Methylation
S201 Phosphorylation
S204 Phosphorylation
T205 Phosphorylation
S243 Phosphorylation P41743 (PRKCI)
T304 Phosphorylation P41743 (PRKCI)
K319 Ubiquitination
K365 Acetylation
K371 Acetylation
T374 Phosphorylation P17612 (PRKACA)
S408 Phosphorylation P54646 (PRKAA2)
K518 Acetylation
S521 Phosphorylation
T527 Phosphorylation
S538 Phosphorylation
S544 Phosphorylation P17612 (PRKACA)
S545 Phosphorylation
S560 Phosphorylation P17612 (PRKACA)
R597 Methylation
S602 Phosphorylation
S622 Phosphorylation
S640 Phosphorylation P17612 (PRKACA)
S934 Phosphorylation
S937 Phosphorylation
S939 Phosphorylation
S968 Phosphorylation
S1071 Phosphorylation
T1074 Phosphorylation P54646 (PRKAA2)
S1078 Phosphorylation

研究背景

功能:

Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.

Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

翻译修饰:

Phosphorylated in vitro by ULK3.

Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.

细胞定位:

Cytoplasm. Nucleus.
Note: Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues.

亚基结构:

Interacts with KIF7 (By similarity). Interacts with STK36. Interacts with ZIC1; the interaction enhances transcription activation. Interacts with SUFU; this inhibits transcriptional activation by GLI1.

蛋白家族:

Belongs to the GLI C2H2-type zinc-finger protein family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

文献引用

1). PRKCI Mediates Radiosensitivity via the Hedgehog/GLI1 Pathway in Cervical Cancer. Frontiers in Oncology, 2023 (PubMed: 35785194) [IF=4.7]

Application: WB    Species: Human    Sample: HeLa cells

Figure 6 PRKCI mediated radiosensitivity in CC via the Hh signaling pathway to change GLI1 localization. (A) The viability of the si-GLI1-transfected HeLa-ov-PRKCI and SiHa-ov-PRKCI cells after radiotherapy at 8 Gy determined by CCK-8 assays. (B) Western blotting assays were used to assess the protein expression of PRKCI when GLI1 expression was downregulated by siRNA in both the HeLa-ov-PRKCI and SiHa-ov-PRKCI cells. (C) The relative protein localization of GLI1 after transfection with shRNA-con and shRNA-PRKCI or ov-con and ov-PRKCI in the HeLa and SiHa cells treated with radiation was determined by immunofluorescence assays. (D) Western blotting analysis of GLI1 and p-GLI1 protein levels in the shRNA-PRKCI- and ov-PRKCI-transfected HeLa and SiHa cells. (E) Western blotting analysis of GLI1 and p-GLI1 protein levels in the cytoplasmic and nuclear fractions of the shRNA-PRKCI- and ov-PRKCI-transfected HeLa and SiHa cells. Scale bars are shown. Data are shown as the mean ± SD from three independent experiments. NS, not significant. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by multiple t-tests (A). CC, cervical cancer; CCK-8, Cell Counting Kit-8.

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