Phospho-TRIM32 (Ser339) Antibody - #AF7074

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产品: 磷酸化 TRIM32 (Ser339) 抗体
货号: AF7074
描述: Rabbit polyclonal antibody to Phospho-TRIM32 (Ser339)
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 72kDa; 72kD(Calculated).
蛋白号: Q13049
RRID: AB_2843514

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-TRIM32 (Ser339) Antibody detects endogenous levels of TRIM32 only when phosphorylated at Ser339.
RRID:
AB_2843514
引用格式: Affinity Biosciences Cat# AF7074, RRID:AB_2843514.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A;

抗原和靶标

免疫原:
Uniprot:

Q13049(TRI32_HUMAN) >>Visit HPA database.

基因/基因ID:
TRIM32(22954)
表达:
Q13049 TRI32_HUMAN:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

序列:
MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13049 作为底物

Site PTM Type Enzyme
S7 Phosphorylation
K50 Ubiquitination
K66 Ubiquitination
S72 Phosphorylation
K139 Ubiquitination
K152 Ubiquitination
K167 Ubiquitination
K175 Ubiquitination
K182 Ubiquitination
K204 Ubiquitination
K215 Ubiquitination
Y241 Phosphorylation
K245 Ubiquitination
K247 Ubiquitination
T274 Phosphorylation
K282 Ubiquitination
S306 Phosphorylation
T321 Phosphorylation
S328 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
K342 Ubiquitination
K359 Ubiquitination
K401 Ubiquitination
K405 Ubiquitination
K471 Ubiquitination
K506 Ubiquitination
S570 Phosphorylation
K604 Ubiquitination
T617 Phosphorylation
K627 Ubiquitination
S651 Phosphorylation

研究背景

功能:

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

翻译修饰:

Ubiquitinated.

细胞定位:

Cytoplasm.
Note: Localized in cytoplasmic bodies, often located around the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

亚基结构:

Interacts with DTNBP1. It self-associates.

蛋白家族:

Belongs to the TRIM/RBCC family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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