| 产品: | 磷酸化 USP22 (Thr147) 抗体 |
| 货号: | AF7076 |
| 描述: | Rabbit polyclonal antibody to Phospho-USP22 (Thr147) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 66kDa; 60kD(Calculated). |
| 蛋白号: | Q9UPT9 |
| RRID: | AB_2843516 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7076, RRID:AB_2843516.
展开/折叠
Deubiquitinating enzyme 22; KIAA1063; Ubiquitin carboxyl terminal hydrolase 22; Ubiquitin carboxyl-terminal hydrolase 22; Ubiquitin specific peptidase 22; Ubiquitin specific peptidase 3 like; Ubiquitin specific processing protease 22; Ubiquitin specific protease 22; Ubiquitin thioesterase 22; Ubiquitin thiolesterase 22; Ubiquitin-specific-processing protease 22; UBP22_HUMAN; USP 22; Usp22; USP3L;
抗原和靶标
Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
- Q9UPT9 UBP22_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSRPEPEGEAMDAELAVAPPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVHLNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UPT9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K35 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K129 | Acetylation | Uniprot | |
| K129 | Sumoylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K137 | Sumoylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| K152 | Sumoylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| R208 | Methylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K382 | Sumoylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Sumoylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| Y450 | Phosphorylation | Uniprot | |
| K462 | Sumoylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| R485 | Methylation | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot |
研究背景
Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
Nucleus.
Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recruitment to the SAGA complex. Interacts with ATXN7L3 and weakly with ATXN7L3B.
Belongs to the peptidase C19 family. UBP8 subfamily.
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