| 产品: | 磷酸化 MyoD1 (Thr115) 抗体 |
| 货号: | AF7079 |
| 描述: | Rabbit polyclonal antibody to Phospho-MyoD1 (Thr115) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 34kDa; 35kD(Calculated). |
| 蛋白号: | P15172 |
| RRID: | AB_2843519 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7079, RRID:AB_2843519.
展开/折叠
bHLHc1; Class C basic helix-loop-helix protein 1; MYF 3; Myf-3; MYF3; Myoblast determination protein 1; Myod 1; MYOD; MYOD1; MYOD1_HUMAN; Myogenic differentiation 1; Myogenic factor 3; Myogenic factor MYF 3; Myogenin D1; PUM;
抗原和靶标
- P15172 MYOD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15172 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y30 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K58 | Acetylation | Uniprot | |
| K99 | Acetylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K104 | Methylation | Uniprot | |
| T115 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y156 | Phosphorylation | Q02750 (MAP2K1) | Uniprot |
| S200 | Phosphorylation | P53778 (MAPK12) , P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S201 | Phosphorylation | P53778 (MAPK12) | Uniprot |
| S204 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot |
研究背景
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
Nucleus.
Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity). Interacts with CSRP3. Interacts with NUPR1 (By similarity).
限制条款
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