| 产品: | 磷酸化 SMYD2 (Ser283) 抗体 |
| 货号: | AF7091 |
| 描述: | Rabbit polyclonal antibody to Phospho-SMYD2 (Ser283) |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 49kDa; 50kD(Calculated). |
| 蛋白号: | Q9NRG4 |
| RRID: | AB_2843531 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7091, RRID:AB_2843531.
展开/折叠
Histone methyltransferase SMYD2; HSKM B; HSKM-B; HSKMB; KMT3C; Lysine N-methyltransferase 3C; MGC119305; N lysine methyltransferase SMYD2; N-lysine methyltransferase SMYD2; SET and MYND domain containing 2; SET and MYND domain containing protein 2; SET and MYND domain-containing protein 2; Smyd2; SMYD2_HUMAN; Zinc finger MYND domain containing 14; ZMYND14;
抗原和靶标
- Q9NRG4 SMYD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLGFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDKDKAKVEIRKLSDPPKAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVCLYMQDWEGALQYGQKIIKPYSKHYPLYSLNVASMWLKLGRLYMGLEHKAAGEKALKKAIAIMEVAHGKDHPYISEIKQEIESH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NRG4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S35 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K398 | Methylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot |
研究背景
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Cytoplasm>Cytosol. Nucleus.
Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1 (By similarity). Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1.
Belongs to the class V-like SAM-binding methyltransferase superfamily.
限制条款
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