产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7113, RRID:AB_2843553.
展开/折叠
FA 1; FA; FA H; FA1; FAA; FACA; FAH; Fanca; FANCA_HUMAN; FANCH; Fanconi anemia complementation group A; Fanconi anemia complementation group H; Fanconi anemia group A protein; Fanconi anemia type 1; MGC75158; Protein FACA;
抗原和靶标
- O15360 FANCA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVTVDVLQRMLIFALDALAAGVQEESSTHKIVRCWFGVFSGHTLGSVISTDPLKRFFSHTLTQILTHSPVLKASDAVQMQREWSFARTHPLLTSLYRRLFVMLSAEELVGHLQEVLETQEVHWQRVLSFVSALVVCFPEAQQLLEDWVARLMAQAFESCQLDSMVTAFLVVRQAALEGPSAFLSYADWFKASFGSTRGYHGCSKKALVFLFTFLSELVPFESPRYLQVHILHPPLVPGKYRSLLTDYISLAKTRLADLKVSIENMGLYEDLSSAGDITEPHSQALQDVEKAIMVFEHTGNIPVTVMEASIFRRPYYVSHFLPALLTPRVLPKVPDSRVAFIESLKRADKIPPSLYSTYCQACSAAEEKPEDAALGVRAEPNSAEEPLGQLTAALGELRASMTDPSQRDVISAQVAVISERLRAVLGHNEDDSSVEISKIQLSINTPRLEPREHMAVDLLLTSFCQNLMAASSVAPPERQGPWAALFVRTMCGRVLPAVLTRLCQLLRHQGPSLSAPHVLGLAALAVHLGESRSALPEVDVGPPAPGAGLPVPALFDSLLTCRTRDSLFFCLKFCTAAISYSLCKFSSQSRDTLCSCLSPGLIKKFQFLMFRLFSEARQPLSEEDVASLSWRPLHLPSADWQRAALSLWTHRTFREVLKEEDVHLTYQDWLHLELEIQPEADALSDTERQDFHQWAIHEHFLPESSASGGCDGDLQAACTILVNALMDFHQSSRSYDHSENSDLVFGGRTGNEDIISRLQEMVADLELQQDLIVPLGHTPSQEHFLFEIFRRRLQALTSGWSVAASLQRQRELLMYKRILLRLPSSVLCGSSFQAEQPITARCEQFFHLVNSEMRNFCSHGGALTQDITAHFFRGLLNACLRSRDPSLMVDFILAKCQTKCPLILTSALVWWPSLEPVLLCRWRRHCQSPLPRELQKLQEGRQFASDFLSPEAASPAPNPDWLSAAALHFAIQQVREENIRKQLKKLDCEREELLVFLFFFSLMGLLSSHLTSNSTTDLPKAFHVCAAILECLEKRKISWLALFQLTESDLRLGRLLLRVAPDQHTRLLPFAFYSLLSYFHEDAAIREEAFLHVAVDMYLKLVQLFVAGDTSTVSPPAGRSLELKGQGNPVELITKARLFLLQLIPRCPKKSFSHVAELLADRGDCDPEVSAALQSRQQAAPDADLSQEPHLF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15360 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K34 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| T351 | Phosphorylation | P51955 (NEK2) | Uniprot |
| S357 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| Y488 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| R683 | Methylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| S858 | Phosphorylation | Uniprot | |
| K921 | Sumoylation | Uniprot | |
| K1079 | Ubiquitination | Uniprot | |
| S1149 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K1199 | Ubiquitination | Uniprot | |
| S1208 | Phosphorylation | Uniprot | |
| S1301 | Phosphorylation | Uniprot | |
| T1309 | Phosphorylation | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| K1398 | Ubiquitination | Uniprot | |
| K1413 | Ubiquitination | Uniprot | |
| S1449 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
研究背景
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.
Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCG and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. Interacts with FAAP20/C1orf86; interaction is direct.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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