Phospho-CTNND1 (Tyr280) Antibody | Affinity Biosciences LTD|亲科生物官网

IHC
IF/ICC
pElisa

1/4

AF7136 at 1/100 staining human kidney tissue sections by IHC-P.

AF7136 at 1/100 staining mouse brain tissue sections by IHC-P.

AF7136 staining H2O2 treated HepG2 cells by IF/ICC.

peptide-ELISA analysis of AF7136.

产品:	磷酸化 CTNND1 (Tyr280) 抗体
货号:	AF7136
描述:	Rabbit polyclonal antibody to Phospho-CTNND1 (Tyr280)
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Horse, Rabbit
分子量:	105kDa; 108kD(Calculated).
蛋白号:	O60716
RRID:	AB_2843576

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2800	现货
200ul	RMB¥ 3800	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%)

克隆:

Polyclonal

特异性:

Phospho-CTNND1 (Tyr280) Antibody detects endogenous levels of CTNND1 only when phosphorylated at Tyr280.

RRID:

AB_2843576
引用格式: Affinity Biosciences Cat# AF7136, RRID:AB_2843576.

偶联:

Unconjugated.

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Cadherin associated Src substrate; Cadherin-associated Src substrate; CAS; Catenin (cadherin associated protein) delta 1; Catenin delta 1; Catenin delta; Catenin delta-1; CTND1_HUMAN; CTNND 1; CTNND; CTNND1; KIAA0384; p120; P120 CAS; p120 catenin; P120 CTN; p120(cas); p120(ctn); P120CAS; P120CTN;

抗原和靶标

免疫原:

Uniprot:

O60716(CTND1_HUMAN) >>Visit HPA database.

基因/基因ID:

CTNND1(1500)

表达:

O60716 CTND1_HUMAN:

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

序列:

O60716 CTND1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Zebrafish

100

Rabbit

100

Xenopus

Sheep

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60716 作为底物

O60716

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S4	Phosphorylation		Uniprot
K25	Ubiquitination		Uniprot
T27	Phosphorylation		Uniprot
S38	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
T59	Phosphorylation		Uniprot
T61	Phosphorylation		Uniprot
K79	Ubiquitination		Uniprot
S81	Phosphorylation		Uniprot
K84	Ubiquitination		Uniprot
Y96	Phosphorylation		Uniprot
S97	Phosphorylation		Uniprot
T98	Phosphorylation		Uniprot
R101	Methylation		Uniprot
Y112	Phosphorylation	P06241 (FYN)	Uniprot
S125	Phosphorylation		Uniprot
S129	Phosphorylation		Uniprot
T133	Phosphorylation		Uniprot
T134	Phosphorylation		Uniprot
K146	Ubiquitination		Uniprot
T147	Phosphorylation		Uniprot
T149	Phosphorylation		Uniprot
T152	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
S171	Phosphorylation		Uniprot
Y174	Phosphorylation		Uniprot
T177	Phosphorylation		Uniprot
K184	Ubiquitination		Uniprot
Y193	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
Y208	Phosphorylation		Uniprot
Y213	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
Y217	Phosphorylation	P12931 (SRC)	Uniprot
Y221	Phosphorylation		Uniprot
S225	Phosphorylation		Uniprot
Y228	Phosphorylation	P00533 (EGFR) , P12931 (SRC)	Uniprot
S230	Phosphorylation		Uniprot
S232	Phosphorylation		Uniprot
T235	Phosphorylation		Uniprot
Y241	Phosphorylation		Uniprot
R242	Methylation		Uniprot
S244	Phosphorylation		Uniprot
Y248	Phosphorylation		Uniprot
R249	Methylation		Uniprot
S252	Phosphorylation		Uniprot
Y257	Phosphorylation		Uniprot
S268	Phosphorylation	Q02156 (PRKCE) , P49674 (CSNK1E)	Uniprot
S269	Phosphorylation		Uniprot
Y280	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
Y291	Phosphorylation		Uniprot
Y296	Phosphorylation		Uniprot
S300	Phosphorylation		Uniprot
Y302	Phosphorylation		Uniprot
T304	Phosphorylation		Uniprot
T308	Phosphorylation		Uniprot
T310	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
S320	Phosphorylation		Uniprot
Y321	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
Y334	Phosphorylation		Uniprot
Y335	Phosphorylation		Uniprot
S346	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K355	Acetylation		Uniprot
K355	Ubiquitination		Uniprot
C394	S-Nitrosylation		Uniprot
Y395	Phosphorylation		Uniprot
K408	Ubiquitination		Uniprot
K421	Ubiquitination		Uniprot
K422	Ubiquitination		Uniprot
K433	Ubiquitination		Uniprot
K444	Ubiquitination		Uniprot
T469	Phosphorylation		Uniprot
K517	Ubiquitination		Uniprot
S539	Phosphorylation		Uniprot
S542	Phosphorylation		Uniprot
K574	Ubiquitination		Uniprot
S587	Phosphorylation		Uniprot
Y588	Phosphorylation		Uniprot
Y600	Phosphorylation		Uniprot
T611	Phosphorylation		Uniprot
S617	Phosphorylation		Uniprot
C618	S-Nitrosylation		Uniprot
K622	Acetylation		Uniprot
K622	Ubiquitination		Uniprot
K623	Acetylation		Uniprot
K633	Ubiquitination		Uniprot
K634	Ubiquitination		Uniprot
T643	Phosphorylation		Uniprot
T650	Phosphorylation		Uniprot
S651	Phosphorylation		Uniprot
Y656	Phosphorylation		Uniprot
Y668	Phosphorylation		Uniprot
S670	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
K676	Ubiquitination		Uniprot
K710	Ubiquitination		Uniprot
S713	Phosphorylation		Uniprot
T720	Phosphorylation		Uniprot
K728	Ubiquitination		Uniprot
S731	Phosphorylation		Uniprot
R735	Methylation		Uniprot
K744	Ubiquitination		Uniprot
K749	Ubiquitination		Uniprot
T774	Phosphorylation		Uniprot
S777	Phosphorylation		Uniprot
K804	Ubiquitination		Uniprot
K810	Ubiquitination		Uniprot
S811	Phosphorylation		Uniprot
K841	Ubiquitination		Uniprot
K846	Ubiquitination		Uniprot
S847	Phosphorylation		Uniprot
S857	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
S861	Phosphorylation		Uniprot
S862	Phosphorylation		Uniprot
S864	Phosphorylation		Uniprot
Y865	Phosphorylation		Uniprot
S868	Phosphorylation		Uniprot
T869	Phosphorylation		Uniprot
K878	Ubiquitination		Uniprot
S879	Phosphorylation	P17252 (PRKCA) , P17612 (PRKACA)	Uniprot
K881	Ubiquitination		Uniprot
K882	Sumoylation		Uniprot
K882	Ubiquitination		Uniprot
S895	Phosphorylation		Uniprot
K898	Ubiquitination		Uniprot
S899	Phosphorylation		Uniprot
Y904	Phosphorylation		Uniprot
S905	Phosphorylation		Uniprot
T906	Phosphorylation		Uniprot
T916	Phosphorylation		Uniprot
S920	Phosphorylation		Uniprot
T932	Phosphorylation		Uniprot
T933	Phosphorylation		Uniprot
S943	Phosphorylation		Uniprot
Y962	Phosphorylation		Uniprot
S964	Phosphorylation		Uniprot

研究背景

O60716_CTND1_HUMAN

功能:

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.

翻译修饰:

Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK5. Dephosphorylated by PTPRJ.

细胞定位:

Cell junction>Adherens junction. Cytoplasm. Nucleus. Cell membrane.
Note: Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).

Nucleus.

Nucleus.

Nucleus.

组织特异性:

亚基结构:

Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin/CDH1, alpha-catenin/CTNNA1, beta-catenin/CTNNB1, and gamma-catenin/JUP. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1. Binds to the C-terminal fragment of PSEN1 and mutually competes for CDH1. Interacts with ZBTB33. Interacts with GLIS2. Interacts with FER. Interacts with NANOS1 (via N-terminal region). Interacts (via N-terminus) with GNA12; the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with GNA13. Interacts with CCDC85B. Interacts with PLPP3; negatively regulates the PLPP3-mediated stabilization of CTNNB1.

蛋白家族:

A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.

ARM repeats 1 to 5 mediate interaction with cadherins.

Belongs to the beta-catenin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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Phospho-CTNND1 (Tyr280) Antibody - #AF7136

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