Phospho-ROCK2 (Ser1366) Antibody - #AF7143
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(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7143, RRID:AB_2843583.
展开/折叠
coiled-coil-containing protein kinase 2; KIAA0619; p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho associated, coiled coil containing protein kinase II; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; ROCK 2; Rock II; Rock2; ROCK2_HUMAN; Rock2m; ROK alpha; ROKalpha;
抗原和靶标
- O75116 ROCK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75116 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T16 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T414 | Phosphorylation | O75116 (ROCK2) | Uniprot |
| S423 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| T567 | Phosphorylation | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| T577 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| K669 | Ubiquitination | Uniprot | |
| K709 | Ubiquitination | Uniprot | |
| K720 | Ubiquitination | Uniprot | |
| Y722 | Phosphorylation | P12931 (SRC) | Uniprot |
| S724 | Phosphorylation | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| K749 | Ubiquitination | Uniprot | |
| K759 | Ubiquitination | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| Y936 | Phosphorylation | Uniprot | |
| S937 | Phosphorylation | Uniprot | |
| K946 | Acetylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K964 | Ubiquitination | Uniprot | |
| T967 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K989 | Ubiquitination | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| K997 | Ubiquitination | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| K1017 | Ubiquitination | Uniprot | |
| K1022 | Ubiquitination | Uniprot | |
| K1071 | Ubiquitination | Uniprot | |
| T1078 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| K1086 | Ubiquitination | Uniprot | |
| S1099 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1114 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Uniprot | |
| S1132 | Phosphorylation | Uniprot | |
| S1133 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1134 | Phosphorylation | Uniprot | |
| S1137 | Phosphorylation | Uniprot | |
| T1165 | Phosphorylation | Uniprot | |
| K1181 | Ubiquitination | Uniprot | |
| S1187 | Phosphorylation | Uniprot | |
| S1194 | Phosphorylation | Uniprot | |
| Y1197 | Phosphorylation | Uniprot | |
| T1212 | Phosphorylation | Uniprot | |
| K1222 | Ubiquitination | Uniprot | |
| Y1232 | Phosphorylation | Uniprot | |
| S1238 | Phosphorylation | Uniprot | |
| K1252 | Ubiquitination | Uniprot | |
| K1316 | Ubiquitination | Uniprot | |
| Y1318 | Phosphorylation | Uniprot | |
| Y1319 | Phosphorylation | Uniprot | |
| T1334 | Phosphorylation | Uniprot | |
| K1339 | Ubiquitination | Uniprot | |
| K1350 | Acetylation | Uniprot | |
| K1351 | Acetylation | Uniprot | |
| K1351 | Ubiquitination | Uniprot | |
| S1361 | Phosphorylation | Uniprot | |
| S1362 | Phosphorylation | Uniprot | |
| T1365 | Phosphorylation | Uniprot | |
| S1366 | Phosphorylation | Uniprot | |
| K1368 | Methylation | Uniprot | |
| K1368 | Ubiquitination | Uniprot | |
| S1374 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1379 | Phosphorylation | Uniprot | |
| S1388 | Phosphorylation | Uniprot |
翻译修饰 - O75116 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O14974 (PPP1R12A) | T443 | Uniprot |
| O14974 (PPP1R12A) | T696 | Uniprot |
| O14974 (PPP1R12A) | S852 | Uniprot |
| O14974-1 (PPP1R12A) | T853 | Uniprot |
| O75116 (ROCK2) | T414 | Uniprot |
| P05067-4 (APP) | T654 | Uniprot |
| P08670 (VIM) | S39 | Uniprot |
| P08670 (VIM) | S72 | Uniprot |
| P15311 (EZR) | T567 | Uniprot |
| P19634 (SLC9A1) | T653 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P26038 (MSN) | T558 | Uniprot |
| P29474 (NOS3) | T495 | Uniprot |
| P35241 (RDX) | T564 | Uniprot |
| P35568 (IRS1) | S636 | Uniprot |
| P35568 (IRS1) | S974 | Uniprot |
| P35611 (ADD1) | T445 | Uniprot |
| P35611 (ADD1) | T480 | Uniprot |
| P51911 (CNN1) | T170 | Uniprot |
| P51911 (CNN1) | S175 | Uniprot |
| P51911 (CNN1) | T180 | Uniprot |
| P51911 (CNN1) | T184 | Uniprot |
| P51911 (CNN1) | T259 | Uniprot |
| P53671 (LIMK2) | T505 | Uniprot |
| P56817 (BACE1) | S498 | Uniprot |
| P68104 (EEF1A1) | T432 | Uniprot |
| Q15306 (IRF4) | S447 | Uniprot |
| Q15306 (IRF4) | S448 | Uniprot |
| Q15417 (CNN3) | S293 | Uniprot |
| Q16555 (DPYSL2) | T555 | Uniprot |
| Q92673 (SORL1) | S2206 | Uniprot |
| Q96A00 (PPP1R14A) | T38 | Uniprot |
| Q96C90 (PPP1R14B) | T57 | Uniprot |
| Q99962 (SH3GL2) | T14 | Uniprot |
研究背景
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
Expressed in the brain (at protein level).
Homodimer (By similarity). Interacts with IRS1 (By similarity). Interacts with RAF1 (By similarity). Interacts with RHOA (activated by GTP), RHOB and RHOC (By similarity). Interacts with PPP1R12A. Interacts with EP300. Interacts with CHORDC1. Interacts with BRCA2. Interacts with NPM1; this interaction enhances ROCK2 activity. Interacts with SORL1.
An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
文献引用
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