| 产品: | 磷酸化 STAM2 (Tyr371) 抗体 |
| 货号: | AF7158 |
| 描述: | Rabbit polyclonal antibody to Phospho-STAM2 (Tyr371) |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 58kDa; 58kD(Calculated). |
| 蛋白号: | O75886 |
| RRID: | AB_2843598 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7158, RRID:AB_2843598.
展开/折叠
DKFZp564C047; Hbp; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; Signal transducing adaptor molecule 2A; Signal transducing adaptor molecule 2B; Signal transducing adaptor molecule; STAM like protein containing SH3 and ITAM domains 2; STAM-2; Stam2; STAM2_HUMAN; STAM2A; STAM2B;
抗原和靶标
- O75886 STAM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQTVSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSELNVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSHGGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSYQNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75886 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K57 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | P06241 (FYN) | Uniprot |
| K296 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| Y371 | Phosphorylation | P06241 (FYN) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| Y374 | Phosphorylation | P06241 (FYN) | Uniprot |
| S375 | Phosphorylation | Uniprot | |
| Y418 | Phosphorylation | Uniprot |
研究背景
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Ubiquitously expressed.
Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity).
The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
The SH3 domain mediates the interaction with USP8.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Belongs to the STAM family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
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