| 产品: | 磷酸化 PAK3 (Ser186) 抗体 |
| 货号: | AF7159 |
| 描述: | Rabbit polyclonal antibody to Phospho-PAK3 (Ser186) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 72kDa; 62kD(Calculated). |
| 蛋白号: | O75914 |
| RRID: | AB_2843599 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7159, RRID:AB_2843599.
展开/折叠
Alpha PAK; Beta PAK; bPAK; CDKN1A; Gamma PAK; hPAK3; MRX30; MRX47; Oligophrenin 3; OPHN3; P21 (CDKN1A) activated kinase 2; P21 (CDKN1A) activated kinase 3; p21 activated kinase 1; p21 activated kinase 2; p21 activated kinase 3; P21 protein (Cdc42/Rac) activated kinase 1; P21 protein (Cdc42/Rac) activated kinase 2; P21 protein (Cdc42/Rac) activated kinase 3; P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast); P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); P58; P65 PAK; PAK 2; PAK 3; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma; S6/H4 kinase; Serine/threonine protein kinase PAK 1; Serine/threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 3; Serine/threonine protein kinase PAK1; Serine/threonine protein kinase PAK2; Serine/threonine protein kinase PAK3; STE20 homolog, yeast;
抗原和靶标
Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
- O75914 PAK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDGLDNEEKPPAPPLRMNSNNRDSSALNHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTPDLYGSQMCPGKLPEGIPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSKETVNNQKYMSFTSGDKSAHGYIAAHPSSTKTASEPPLAPPVSEEEDEEEEEEEDENEPPPVIAPRPEHTKSIYTRSVVESIASPAVPNKEVTPPSAENANSSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLAKPLSSLTPLIIAAKEAIKNSSR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75914 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| K257 | Acetylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| K269 | Acetylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| T428 | Phosphorylation | Uniprot | |
| K433 | Acetylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| Y477 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K535 | Acetylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S558 | Phosphorylation | Uniprot |
翻译修饰 - O75914 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O75914-2 (PAK3) | S139 | Uniprot |
| O75914-2 (PAK3) | T421 | Uniprot |
| P04049 (RAF1) | S338 | Uniprot |
| P04049 (RAF1) | S339 | Uniprot |
| P17600-1 (SYN1) | S605 | Uniprot |
| P19429 (TNNI3) | S150 | Uniprot |
| Q02750 (MAP2K1) | S298 | Uniprot |
| Q05682 (CALD1) | S714 | Uniprot |
| Q05682 (CALD1) | S744 | Uniprot |
| Q16659 (MAPK6) | S189 | Uniprot |
| Q9UM54-1 (MYO6) | T405 | Uniprot |
研究背景
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
Autophosphorylated when activated by CDC42/p21.
Neddylated.
Cytoplasm.
Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK. Interacts with the C-terminal of APP (By similarity). Interacts with ARHGEF6 and ARHGEF7.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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