产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7164, RRID:AB_2843604.
展开/折叠
AA415432; FLJ21151; GG10 2; GG10_2; KI20A_HUMAN; KIF20A; kinesin family member 20A; Kinesin like protein KIF20A; Kinesin-like protein 174; Kinesin-like protein KIF20A; mitotic kinesin like protein 2; Mitotic kinesin-like protein 2; MKlp2; OTTHUMP00000159481; RAB6 interacting, kinesin like (rabkinesin6); Rab6-interacting kinesin-like protein; RAB6-INTERACTING PROTEIN, KINESIN-LIKE; RAB6KIFL; Rabkinesin 6; Rabkinesin-6;
抗原和靶标
- O95235 KI20A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95235 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| Y558 | Phosphorylation | Uniprot | |
| T572 | Phosphorylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| Y637 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| S677 | Phosphorylation | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| T686 | Phosphorylation | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| T706 | Phosphorylation | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| K739 | Ubiquitination | Uniprot | |
| K750 | Ubiquitination | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| T832 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot | |
| T859 | Phosphorylation | Uniprot | |
| S862 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| T864 | Phosphorylation | Uniprot | |
| S867 | Phosphorylation | Uniprot | |
| Y869 | Phosphorylation | Uniprot | |
| S875 | Phosphorylation | Uniprot | |
| S878 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S883 | Phosphorylation | Uniprot |
研究背景
Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
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