产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7220, RRID:AB_2843660.
展开/折叠
EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26;
抗原和靶标
- P13639 EF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P13639 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R10 | Methylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| C41 | S-Nitrosylation | Uniprot | |
| K42 | Acetylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| T54 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| T57 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| T59 | Phosphorylation | O00418 (EEF2K) | Uniprot |
| C67 | S-Nitrosylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| Y79 | Phosphorylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K239 | Acetylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K252 | Acetylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K258 | Acetylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| R264 | Methylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Methylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| T288 | Phosphorylation | Uniprot | |
| C290 | S-Nitrosylation | Uniprot | |
| K308 | Acetylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K314 | Acetylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K318 | Acetylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K322 | Acetylation | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K337 | Acetylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| C369 | S-Nitrosylation | Uniprot | |
| Y373 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K391 | Acetylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| K407 | Acetylation | Uniprot | |
| K407 | Methylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| R409 | Methylation | Uniprot | |
| Y411 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| K426 | Acetylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| R428 | Methylation | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K438 | Acetylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K439 | Acetylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Methylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T450 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| R495 | Methylation | Uniprot | |
| K498 | Acetylation | Uniprot | |
| K498 | Methylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K519 | Methylation | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| K525 | Methylation | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| R526 | Methylation | Uniprot | |
| K529 | Sumoylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| C567 | S-Nitrosylation | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| Y579 | Phosphorylation | Uniprot | |
| T582 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| C591 | S-Nitrosylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| K594 | Methylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S595 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K598 | Ubiquitination | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| K638 | Acetylation | Uniprot | |
| K638 | Methylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K648 | Acetylation | Uniprot | |
| K648 | Ubiquitination | Uniprot | |
| C651 | S-Nitrosylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| Y671 | Phosphorylation | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| K688 | Acetylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| R698 | Methylation | Uniprot | |
| R716 | Methylation | Uniprot | |
| T724 | Phosphorylation | Uniprot | |
| R726 | Methylation | Uniprot | |
| C728 | S-Nitrosylation | Uniprot | |
| Y730 | Phosphorylation | Uniprot | |
| S732 | Phosphorylation | Uniprot | |
| R739 | Methylation | Uniprot | |
| Y745 | Phosphorylation | Uniprot | |
| Y760 | Phosphorylation | Uniprot | |
| K766 | Ubiquitination | Uniprot | |
| R767 | Methylation | Uniprot | |
| T779 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| S802 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| K845 | Ubiquitination | Uniprot | |
| K857 | Acetylation | Uniprot | |
| K857 | Ubiquitination | Uniprot |
研究背景
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).
(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated.
Proteolytically processed at two sites following phosphorylation by CSK.
SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Cytoplasm. Nucleus.
Note: Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with RBPMS2.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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