| 产品: | 磷酸化 HCLS1 (Tyr198) 抗体 |
| 货号: | AF7226 |
| 描述: | Rabbit polyclonal antibody to Phospho-HCLS1 (Tyr198) |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 78kDa; 54kD(Calculated). |
| 蛋白号: | P14317 |
| RRID: | AB_2843666 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7226, RRID:AB_2843666.
展开/折叠
Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; HS1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75;
抗原和靶标
- P14317 HCLS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGTRGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVAVYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P14317 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T23 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S32 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K123 | Acetylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| R155 | Methylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | P07948 (LYN) , P09769 (FGR) | Uniprot |
| K223 | Acetylation | Uniprot | |
| K224 | Acetylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K241 | Acetylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| Y378 | Phosphorylation | P07948 (LYN) , P43405 (SYK) | Uniprot |
| Y397 | Phosphorylation | P09769 (FGR) , P07948 (LYN) , P43405 (SYK) | Uniprot |
| Y481 | Phosphorylation | Uniprot |
研究背景
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.
Membrane>Peripheral membrane protein. Cytoplasm. Mitochondrion.
Expressed only in tissues and cells of hematopoietic origin.
Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS (By similarity). Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54 (By similarity).
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
限制条款
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