产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7243, RRID:AB_2843683.
展开/折叠
90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;
抗原和靶标
- P17480 UBF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17480 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T117 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K126 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y207 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Methylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K254 | Methylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K385 | Acetylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S392 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| R429 | Methylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| R443 | Methylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| Y456 | Phosphorylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| K559 | Acetylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| R642 | Methylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| Y648 | Phosphorylation | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot |
研究背景
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Phosphorylated and activated by PIK3CA.
Nucleus>Nucleolus.
Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.
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