| 产品: | 磷酸化 EIF2S2 (Tyr298) 抗体 |
| 货号: | AF7255 |
| 描述: | Rabbit polyclonal antibody to Phospho-EIF2S2 (Tyr298) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 40KD; 38kD(Calculated). |
| 蛋白号: | P20042 |
| RRID: | AB_2843695 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7255, RRID:AB_2843695.
展开/折叠
DKFZp686L18198; eIF 2 beta; eIF-2-beta; EIF2; EIF2B; EIF2beta; EIF2S2; Eukaryotic initiation factor 2 beta; eukaryotic initiation factor 2-beta; Eukaryotic translation initiation factor 2 beta; Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa; Eukaryotic translation initiation factor 2 subunit 2 beta; Eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; IF2B_HUMAN; MGC8508; PPP1R67; protein phosphatase 1, regulatory subunit 67;
抗原和靶标
- P20042 IF2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P20042 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K79 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| K124 | Methylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| R172 | Methylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S218 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| C226 | S-Nitrosylation | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K265 | Acetylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K276 | Acetylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| K293 | Acetylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| R296 | Methylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K324 | Acetylation | Uniprot | |
| K324 | Ubiquitination | Uniprot |
研究背景
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity).
Belongs to the eIF-2-beta/eIF-5 family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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