Phospho-GATA2 (Ser401) Antibody - #AF7270

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产品: 磷酸化 GATA2 (Ser401) 抗体
货号: AF7270
描述: Rabbit polyclonal antibody to Phospho-GATA2 (Ser401)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Bovine, Horse, Rabbit, Chicken, Xenopus
分子量: 49kDa; 51kD(Calculated).
蛋白号: P23769
RRID: AB_2843710

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Bovine(80%), Horse(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-GATA2 (Ser401) Antibody detects endogenous levels of GATA2 only when phosphorylated at Ser401.
RRID:
AB_2843710
引用格式: Affinity Biosciences Cat# AF7270, RRID:AB_2843710.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DCML; Endothelial transcription factor GATA 2; Endothelial transcription factor GATA-2; Endothelial transcription factor GATA2; FLJ45948; GATA 2; GATA binding protein 2; GATA-binding protein 2; Gata2; GATA2_HUMAN; IMD21; MGC2306; MONOMAC; NFE 1B; NFE1B; OTTHUMP00000216240;

抗原和靶标

免疫原:
Uniprot:

P23769(GATA2_HUMAN) >>Visit HPA database.

基因/基因ID:
GATA2(2624)
表达:
P23769 GATA2_HUMAN:

Endothelial cells.

序列:
MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTCCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSNKSKKSKKGAECFEELSKCMQEKSSPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
80
Pig
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23769 作为底物

Site PTM Type Enzyme
S71 Phosphorylation
S73 Phosphorylation
R86 Methylation
S119 Phosphorylation
T176 Phosphorylation P06493 (CDK1)
S182 Phosphorylation
S186 Phosphorylation
S192 Phosphorylation
Y213 Phosphorylation
S216 Phosphorylation
S220 Phosphorylation
S276 Phosphorylation
S290 Phosphorylation
Y314 Phosphorylation
Y322 Phosphorylation
Y376 Phosphorylation
K389 Sumoylation
S401 Phosphorylation P31749 (AKT1)
K409 Ubiquitination

研究背景

功能:

Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Endothelial cells.

亚基结构:

Interacts with BRD3 (By similarity). Interacts with AR and CCAR1.

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