产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7291, RRID:AB_2843731.
展开/折叠
DKFZp686I18166; EC 2.7.10.2; FLJ16446; MGC105683; OTTHUMP00000231338; OTTHUMP00000231339; Wee 1; WEE 1 homolog 1 (S. pombe); WEE1; WEE1 homolog (S. pombe); Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1, S. pombe, homolog of; WEE1, somatic; Wee1-like protein kinase; WEE1_HUMAN; WEE1A; Wee1A kinase; WEE1hu;
抗原和靶标
- P30291 WEE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30291 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| R14 | Methylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S67 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S123 | Phosphorylation | P53350 (PLK1) , P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S127 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S212 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| T217 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| K588 | Ubiquitination | Uniprot | |
| K590 | Sumoylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| R617 | Methylation | Uniprot | |
| S642 | Phosphorylation | Q8IWQ3 (BRSK2) , Q8TDC3 (BRSK1) , P31749 (AKT1) | Uniprot |
| Y646 | Phosphorylation | Uniprot |
翻译修饰 - P30291 作为激酶
研究背景
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Dephosphorylated at Thr-239 by CTDP1.
Ubiquitinated and degraded at the onset of G2/M phase.
Nucleus.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.