产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7294, RRID:AB_2843734.
展开/折叠
19G; AI325074; AU045576; CDC 21; CDC 54; CDC21; CDC21 homolog; CDC21, S. pombe, homolog of; CDC54; Cell division cycle 21, S. pombe, homolog of; DNA replication licensing factor MCM 4; DNA replication licensing factor MCM4; hCdc 21; hCdc21; Homolog of S. pombe cell devision cycle 21; KIAA4003; mcdc21; MCM 4; MCM 4 minichromosome maintenance deficient 4; mcm4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae); MCM4_HUMAN; Mcmd4; MGC33310; Minichromosome maintenance 4; Minichromosome maintenance complex component 4; Minichromosome maintenance deficient (S. cerevisiae) 4; Minichromosome maintenance deficient 4; Minichromosome maintenance deficient 4 homolog (S. cerevisiae); Minichromosome maintenance, S. cerevisiae, homolog of, 4; mKIAA4003; NKCD; NKGCD; P1 CDC21; P1-CDC21; P1CDC21;
抗原和靶标
- P33991 MCM4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33991 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S3 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S6 | Phosphorylation | Uniprot | |
| T7 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| S9 | Phosphorylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| R11 | Methylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| R15 | Methylation | Uniprot | |
| R17 | Methylation | Uniprot | |
| T19 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| T23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S54 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S61 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T91 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| R108 | Methylation | Uniprot | |
| T110 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| S120 | Phosphorylation | Uniprot | |
| K123 | Acetylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y193 | Phosphorylation | Uniprot | |
| K216 | Methylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| R387 | Methylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K413 | Methylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K439 | Acetylation | Uniprot | |
| K439 | Sumoylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K450 | Acetylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K477 | Sumoylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K478 | Ubiquitination | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| Y522 | Phosphorylation | Uniprot | |
| Y524 | Phosphorylation | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| K627 | Acetylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| Y714 | Phosphorylation | Uniprot | |
| R724 | Methylation | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| K752 | Ubiquitination | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| T788 | Phosphorylation | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| K806 | Methylation | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K819 | Acetylation | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| R828 | Methylation | Uniprot | |
| K837 | Ubiquitination | Uniprot | |
| K858 | Acetylation | Uniprot | |
| K858 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro).
Nucleus.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with MCMBP.
Belongs to the MCM family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
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