产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7304, RRID:AB_2843744.
展开/折叠
Eczema thrombocytopenia; IMD2; SCNX; THC; THC1; Thrombocytopenia 1 (X linked); U42471; Was; WASp; WASP_HUMAN; Wiskott Aldrich syndrome (eczema thrombocytopenia); Wiskott Aldrich syndrome; Wiskott Aldrich syndrome protein; Wiskott-Aldrich syndrome protein;
抗原和靶标
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
- P42768 WASP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P42768 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K76 | Sumoylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y102 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| K144 | Sumoylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Q07912 (TNK2) | Uniprot |
| T282 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| Y291 | Phosphorylation | P07948 (LYN) , P06241 (FYN) , P00519 (ABL1) , Q07912 (TNK2) , Q06187 (BTK) , P06239 (LCK) , P08631 (HCK) | Uniprot |
| S320 | Phosphorylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| R328 | Methylation | Uniprot | |
| R338 | Methylation | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| R364 | Methylation | Uniprot | |
| R377 | Methylation | Uniprot | |
| R431 | Methylation | Uniprot | |
| R439 | Methylation | Uniprot | |
| S483 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) | Uniprot |
| S484 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
研究背景
Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Cytoplasm>Cytoskeleton. Nucleus.
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Binds the Arp2/3 complex. Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Interacts (via C-terminus) with ALDOA. Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with FCHSD2 (By similarity).
(Microbial infection) Interacts with E.coli effector protein EspF(U).
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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