产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7315, RRID:AB_2843755.
展开/折叠
39 kDa protein kinase; CAK; CAK1; Cdk activating kinase; CDK-activating kinase 1; CDK-activating kinase; cdk7; CDK7_HUMAN; CDKN7; Cell division protein kinase 7; Cyclin dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); Cyclin-dependent kinase 7; HCAK; Homolog of Xenopus MO15 Cdk activating kinase; Kinase subunit of CAK; MO15; MO15, Xenopus, homolog of; P39 Mo15; p39MO15; Serine threonine kinase Stk1; Serine/threonine protein kinase 1; Serine/threonine protein kinase MO15; STK1; TFIIH basal transcription factor complex kinase subunit;
抗原和靶标
- P50613 CDK7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALDVKSRAKRYEKLDFLGEGQFATVYKARDKNTNQIVAIKKIKLGHRSEAKDGINRTALREIKLLQELSHPNIIGLLDAFGHKSNISLVFDFMETDLEVIIKDNSLVLTPSHIKAYMLMTLQGLEYLHQHWILHRDLKPNNLLLDENGVLKLADFGLAKSFGSPNRAYTHQVVTRWYRAPELLFGARMYGVGVDMWAVGCILAELLLRVPFLPGDSDLDQLTRIFETLGTPTEEQWPDMCSLPDYVTFKSFPGIPLHHIFSAAGDDLLDLIQGLFLFNPCARITATQALKMKYFSNRPGPTPGCQLPRPNCPVETLKEQSNPALAIKRKRTEALEQGGLPKKLIF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50613 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
| S164 | Phosphorylation | P50613 (CDK7) , P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| Y169 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | P50613 (CDK7) , P41743 (PRKCI) , P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| T175 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T287 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| T332 | Phosphorylation | Uniprot |
翻译修饰 - P50613 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P03372 (ESR1) | S118 | Uniprot |
| P04637 (TP53) | S33 | Uniprot |
| P04637 (TP53) | S371 | Uniprot |
| P04637 (TP53) | S376 | Uniprot |
| P04637 (TP53) | S378 | Uniprot |
| P04637 (TP53) | S392 | Uniprot |
| P06493 (CDK1) | T161 | Uniprot |
| P10275 (AR) | S516 | Uniprot |
| P10276 (RARA) | S77 | Uniprot |
| P11802 (CDK4) | T172 | Uniprot |
| P13631 (RARG) | S77 | Uniprot |
| P13631 (RARG) | S79 | Uniprot |
| P14859 (POU2F1) | S385 | Uniprot |
| P21127 (CDK11B) | T595 | Uniprot |
| P24928 (POLR2A) | S1616 | Uniprot |
| P24928 (POLR2A) | S1619 | Uniprot |
| P24928 (POLR2A) | S1621 | Uniprot |
| P24928 (POLR2A) | S1626 | Uniprot |
| P24928 (POLR2A) | S1647 | Uniprot |
| P24928 (POLR2A) | S1654 | Uniprot |
| P24928 (POLR2A) | S1668 | Uniprot |
| P24928 (POLR2A) | S1675 | Uniprot |
| P24928 (POLR2A) | S1696 | Uniprot |
| P24928 (POLR2A) | S1717 | Uniprot |
| P24928 (POLR2A) | S1724 | Uniprot |
| P24928 (POLR2A) | S1738 | Uniprot |
| P24928 (POLR2A) | S1766 | Uniprot |
| P24928 (POLR2A) | S1787 | Uniprot |
| P24928 (POLR2A) | S1864 | Uniprot |
| P24928 (POLR2A) | S1871 | Uniprot |
| P24928 (POLR2A) | S1878 | Uniprot |
| P24928 (POLR2A) | S1892 | Uniprot |
| P24928 (POLR2A) | S1899 | Uniprot |
| P24928 (POLR2A) | S1913 | Uniprot |
| P24928 (POLR2A) | S1920 | Uniprot |
| P24928 (POLR2A) | S1934 | Uniprot |
| P24928 (POLR2A) | S1944 | Uniprot |
| P24928 (POLR2A) | S1951 | Uniprot |
| P24941 (CDK2) | S46 | Uniprot |
| P24941 (CDK2) | T160 | Uniprot |
| P24941 (CDK2) | T165 | Uniprot |
| P24941 (CDK2) | Y168 | Uniprot |
| P37231 (PPARG) | S112 | Uniprot |
| P49736 (MCM2) | S4 | Uniprot |
| P49736 (MCM2) | S5 | Uniprot |
| P49736 (MCM2) | S7 | Uniprot |
| P49736 (MCM2) | S13 | Uniprot |
| P49736 (MCM2) | S27 | Uniprot |
| P49736 (MCM2) | S40 | Uniprot |
| P49736 (MCM2) | S41 | Uniprot |
| P49736 (MCM2) | S53 | Uniprot |
| P49736 (MCM2) | T59 | Uniprot |
| P49736 (MCM2) | S108 | Uniprot |
| P49736 (MCM2) | S139 | Uniprot |
| P50613 (CDK7) | S164 | Uniprot |
| P50613 (CDK7) | T170 | Uniprot |
| Q00534 (CDK6) | T177 | Uniprot |
| Q01094 (E2F1) | S403 | Uniprot |
| Q01094 (E2F1) | T433 | Uniprot |
| Q13285 (NR5A1) | S203 | Uniprot |
| Q14847-1 (LASP1) | S146 | Uniprot |
| Q15797 (SMAD1) | S206 | Uniprot |
| Q9BYE7-1 (PCGF6) | S30 | Uniprot |
| Q9H0D6 (XRN2) | T439 | Uniprot |
| Q9NYV4 (CDK12) | T893 | Uniprot |
| Q9UPZ9 (ICK) | T157 | Uniprot |
研究背景
Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.
Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.
Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides.
Ubiquitous.
Associates primarily with cyclin-H (CCNH) and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor; this complex is sensitive to UV light. The CAK complex binds to p53/TP53 in response to DNA damage. Interacts with CDK2, SF1/NR5A1, PUF60 and PRKCI.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
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