Phospho-GAB1 (Tyr406) Antibody - #AF7372

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产品: 磷酸化 GAB1 (Tyr406) 抗体
货号: AF7372
描述: Rabbit polyclonal antibody to Phospho-GAB1 (Tyr406)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 76.6kDa; 77kD(Calculated).
蛋白号: Q13480
RRID: AB_2843812

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Phospho-GAB1 (Tyr406) Antibody detects endogenous levels of GAB1 only when phosphorylated at Tyr406.
RRID:
AB_2843812
引用格式: Affinity Biosciences Cat# AF7372, RRID:AB_2843812.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gab 1; GAB1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1;

抗原和靶标

免疫原:
Uniprot:

Q13480(GAB1_HUMAN) >>Visit HPA database.

基因/基因ID:
GAB1(2549)
序列:
MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDAGLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPLAINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSETDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEADGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHPAHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSLEGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVPPPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARDSSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDLDLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13480 作为底物

Site PTM Type Enzyme
S2 Acetylation
Y47 Phosphorylation
Y48 Phosphorylation
Y83 Phosphorylation
Y183 Phosphorylation
K205 Ubiquitination
C213 S-Nitrosylation
K221 Ubiquitination
Y242 Phosphorylation P12931 (SRC) , P06213 (INSR)
S244 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
Y259 Phosphorylation P12931 (SRC)
Y265 Phosphorylation
S266 Phosphorylation
S274 Phosphorylation
Y285 Phosphorylation P00533 (EGFR) , P06213 (INSR) , P08581 (MET)
S304 Phosphorylation
S306 Phosphorylation
Y307 Phosphorylation P00533 (EGFR) , P08581 (MET)
T312 Phosphorylation P28482 (MAPK1)
T316 Phosphorylation
Y317 Phosphorylation P12931 (SRC)
S355 Phosphorylation
T365 Phosphorylation
S367 Phosphorylation
T369 Phosphorylation
S371 Phosphorylation
S372 Phosphorylation
Y373 Phosphorylation P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P12931 (SRC)
S381 Phosphorylation P28482 (MAPK1)
S383 Phosphorylation
T387 Phosphorylation
S389 Phosphorylation
S401 Phosphorylation
S402 Phosphorylation
Y406 Phosphorylation P00533 (EGFR) , P08581 (MET)
S417 Phosphorylation
S418 Phosphorylation
S419 Phosphorylation
Y447 Phosphorylation P06213 (INSR) , P08581 (MET) , P00533 (EGFR)
S454 Phosphorylation P28482 (MAPK1)
Y472 Phosphorylation P00533 (EGFR) , P06213 (INSR) , P08581 (MET)
T476 Phosphorylation P28482 (MAPK1)
S500 Phosphorylation
T503 Phosphorylation
S551 Phosphorylation
S582 Phosphorylation P28482 (MAPK1)
Y589 Phosphorylation P06213 (INSR) , P00533 (EGFR) , P08581 (MET)
S597 Phosphorylation P28482 (MAPK1)
Y627 Phosphorylation P06213 (INSR) , P12931 (SRC) , P08581 (MET) , P09619 (PDGFRB) , P00533 (EGFR)
S634 Phosphorylation
S637 Phosphorylation
T638 Phosphorylation
S645 Phosphorylation
S651 Phosphorylation
Y659 Phosphorylation P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P09619 (PDGFRB)
T673 Phosphorylation
S683 Phosphorylation
T684 Phosphorylation

研究背景

功能:

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.

翻译修饰:

Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

亚基结构:

Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Part of a tripartite complex containing GAB1, METTL13 and SPRY2. Interacts with METTL13.

蛋白家族:

Belongs to the GAB family.

研究领域

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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