产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7394, RRID:AB_2843834.
展开/折叠
bHLHe75; Class E basic helix-loop-helix protein 75; glucocorticoid receptor interacting protein 1; GRIP 1; GRIP1; hTIF2; MED1; MGC138808; NCoA 2; NCoA-2; Ncoa2; NCOA2_HUMAN; Nuclear receptor coactivator 2; Oncogene FGR; p160; SRC 2; src2; Steroid receptor coactivator 2; TIF 2; TIF2; Transcriptional intermediary factor 2;
抗原和靶标
- Q15596 NCOA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLSSNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRHRMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAIINDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGMIRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQDSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLRLQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRTLMMRGQGLNMTPSMVAPSGMPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMAHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGGMSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15596 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | P68400 (CSNK2A1) , P50750 (CDK9) | Uniprot |
| S471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| R489 | Methylation | Uniprot | |
| S493 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| S499 | Phosphorylation | P50750 (CDK9) , P68400 (CSNK2A1) | Uniprot |
| R501 | Methylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| K636 | Acetylation | Uniprot | |
| K640 | Acetylation | Uniprot | |
| K640 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| K705 | Methylation | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K713 | Acetylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| T729 | Phosphorylation | Uniprot | |
| K731 | Sumoylation | Uniprot | |
| S736 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14) | Uniprot |
| K738 | Acetylation | Uniprot | |
| K739 | Acetylation | Uniprot | |
| Y747 | Phosphorylation | Uniprot | |
| K765 | Sumoylation | Uniprot | |
| S771 | Phosphorylation | Uniprot | |
| T773 | Phosphorylation | Uniprot | |
| K780 | Acetylation | Uniprot | |
| K785 | Acetylation | Uniprot | |
| K785 | Sumoylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K788 | Sumoylation | Uniprot | |
| T847 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| T854 | Phosphorylation | Uniprot | |
| R874 | Methylation | Uniprot | |
| K1129 | Sumoylation | Uniprot | |
| R1162 | Methylation | Uniprot | |
| R1169 | Methylation | Uniprot | |
| R1173 | Methylation | Uniprot | |
| R1177 | Methylation | Uniprot | |
| R1190 | Methylation | Uniprot | |
| R1196 | Methylation | Uniprot | |
| R1203 | Methylation | Uniprot | |
| R1221 | Methylation | Uniprot | |
| R1240 | Methylation | Uniprot | |
| R1248 | Methylation | Uniprot | |
| R1250 | Methylation | Uniprot | |
| R1261 | Methylation | Uniprot | |
| R1266 | Methylation | Uniprot | |
| K1454 | Sumoylation | Uniprot |
研究背景
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
Nucleus.
Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3.
Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
Belongs to the SRC/p160 nuclear receptor coactivator family.
研究领域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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