Phospho-SF1 (Ser80/Ser82) Antibody - #AF7395
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7395, RRID:AB_2843835.
展开/折叠
BBP; D11S636; Mammalian branch point binding protein mBBP; OTTHUMP00000069749; OTTHUMP00000069750; OTTHUMP00000069752; Splicing factor 1; Transcription factor ZFM1; ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZNF162; SF1;
抗原和靶标
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
- Q15637 SF01_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15637 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| S82 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
| Y87 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| R263 | Methylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| C279 | S-Nitrosylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| K306 | Acetylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| R345 | Methylation | Uniprot | |
| R365 | Methylation | Uniprot |
研究背景
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Nucleus.
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Interacts with RBM17.
Belongs to the BBP/SF1 family.
限制条款
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