产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7403, RRID:AB_2843843.
展开/折叠
ESP 2; ESP2; HED 2; HED2; ZYX; ZYX protein; Zyxin 2; Zyxin2;
抗原和靶标
- Q15942 ZYX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15942 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R5 | Methylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K25 | Methylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S143 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K165 | Methylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| K201 | Methylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| K265 | Acetylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Methylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K279 | Acetylation | Uniprot | |
| K279 | Methylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T306 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S308 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S313 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| R343 | Methylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K528 | Acetylation | Uniprot |
研究背景
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Cell junction>Focal adhesion.
Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.
Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Interacts with SYNPO2.
(Microbial infection) Interacts with human papillomavirus type 6/HPV6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18.
Belongs to the zyxin/ajuba family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
限制条款
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